Figures & data
Figure 1. The expression of ACE at mRNA level in tumour tissues and cell lines. (A) The ACE expression was higher in tumour tissues compared with in adjacent normal tissues and healthy tissues. (B) The ACE expression was higher in HT-29 cell lines than in FHC cell lines. **p < .05 represented the significant difference between the two.
Figure 2. The methylation status of ACE in tumour tissues and cell lines. (A,B) The methylation of ACE was lower in tumour tissues than in adjacent normal tissues and healthy tissues. (B) The methylation of ACE was lower in HT-29cell lines than in FHC cell lines. **p < .05 represented the significant difference between the two.
Figure 3. The hypomethylation of ACE promoted cell proliferation.
Figure 4. The hypomethylation of ACE suppressed the cell apoptosis. **p < .05 represented the significant difference between the two.
Figure 5. The hypomethylation of ACE promoted the cell colony formation.
Table 1. The relationship between the methylation of ACE gene and clinicopathologic characteristics.
Figure 6. Kaplan–Meier analysis of ACE hypomethylation in patients with CRC. The patients with ACE hypomethylation had a shorter overall survival compared with those with ACE hypermethylation and unmethylation (Log-rank test, p < .001).
Table 2. Multivariate analysis for the prognostic factors in patients with CRC.