Figures & data
Figure 1. Knockdown of LSINCT5 repressed the growth and metastasis of GL15 cells. (A) LSINCT5 expression in 56 glioma tissues and 16 normal controls was detected using qRT-PCR. (B) LSINCT5 expression was analyzed using qRT-PCR after transfection with sh-LSINCT5 #1 and #2. After transfection, (C) cell viability was examined using CCK-8 assay, (D,E) apoptosis was examined using Annexin V-FITC/PI double staining method, and (F) cell migration and invasion were examined using transwell assay. (G) Relative migration and (H) invasion based on the results obtained from transwell assay. *p < .05, **p < .01, ***p < .001.
Figure 2. LSINCT5 acted as a molecular sponge for miR-451. (A) miR-451 expression was examined using qRT-PCR after GL15 cells transfected with sh-LSINCT5 #1 or shNC. (B) LSINCT5 expression was examined using qRT-PCR after GL15 cells transfected with miR-451 mimic or mimic NC. (C) The luciferase activity in GL15 cells co-transfected with mIR-451 mimic and luciferase reporters containing LSINCT5-wt or LSINCT5-mt vector. The results were obtained by dual-luciferase reporter assay. *p < .05, ***p < .001.
Figure 3. LSINCT5 suppressed GL15 cells via regulation of miR-451. (A) miR-451 expression in 56 glioma tissues and 16 normal controls was detected using qRT-PCR. (B) The cell viability, (C,D) cell apoptosis, (E) cell migration and invasion were determined when GL15 cells were transfected with sh-LSINCT5, miR-451 inhibitor, or their negative controls (shNC and inhibitor NC). (F) Relative migration and (G) invasion based on the results obtained from transwell assay. *p < .05, **p < .01, ***p < .001.
Figure 4. Rac1 was a direct target of miR-451. (A) The mRNA and (B) protein levels of Rac1 after transfection with miR-451 mimic, miR-451 inhibitor or the negative controls (mimic NC and inhibitor NC). (C) Luciferase activity in GL15 glioma cells co-transfected with miR-451 mimic and luciferase reporters containing Rac1-wt or Rac1-mt transcript. *p < .05, **p < .01, ***p < .001.
Figure 5. miR-451 suppressed GL15 cells via negative regulation of Rac1. (A) The mRNA and (B) protein levels of Rac1 after transfection with pEX-Rac1 and sh-Rac1. (C) The cell viability, (D,E) cell apoptosis, (F) cell migration and invasion were measured after GL15 cells were transfected with miR-451 mimic, pEX-Rac1, or their negative controls (mimic NC and pEX). (G) Relative migration and (H) invasion based on the results obtained from transwell assay. *p < .05, **p < .01, ***p < .001.
Figure 6. Activation of PI3K/AKT, Wnt/β-catenin and NF-κB pathways were affected by LSINCT5-miR-451-Rac1 axis. (A,B) After transfection with sh-LSINCT5 #1, miR-451 mimic, pEX-Rac1, sh-Rac1 or their respective controls, the protein accumulation of main components of PI3K/AKT, Wnt/β-catenin and NF-κB pathways were analyzed using Western blot.
