Figures & data
Table 1. Primers and sequences of RT-qPCR.
Figure 1. Expression of miR-34a and HNF4α in neuroblastoma tissue. (A) Expression of miR-34a and HNF4α mRNA in human neuroblastoma tissues and normal adjacent tissues; (B) gel electrophoresis of HNF4α protein expression; (C) the expression level of HNF4 proteins in human neuroblastoma and normal adjacent tissues. Note: Compared with the non-tumour tissue group, *p < .05.
Figure 2. Overexpression of miR-34a inhibited the proliferation, migration and invasion of SH-SY5Y cells. (A) Expression of miR-34a in SH-SY5Y cells; (B) overexpression of miR-34a inhibited proliferation of SH-SY5Y cells; (C) the detection of cell migration and invasion after overexpression of miR-34a; (D) overexpression of miR-34a inhibited migration and invasion of SH-SY5Y cells. Note: Compared with the miR-NC group, *p < .05.
Figure 3. Knocking down HNF4α inhibited proliferation, migration and invasion of SH-SY5Y cells. (A) Gel electrophoresis of HNF4α protein expression; (B) expression level of HNF4α protein; (C) knocking down HNF4α inhibited SH-SY5Y cell proliferation; (D) knocking down HNF4α inhibited migration and invasion of SH-SY5Y cells. Note: Compared with the si-NC group, *p < .05.
Figure 4. Overexpression of miR-34a or knockdown of HNF4α inhibited MMP-2 and MMP-14 expression in SH-SY5Y cells. (A) mRNA expression of MMP-2 and MMP-14 in SH-SY5Y cells; (B) MMP-2 and MMP-14 protein electrophoresis in SH-SY5Y cells; (C) expression levels of MMP-2 and MMP-14 proteins in SH-SY5Y cells. Note: Compared with the miR-NC group,*p < .05; compared with si-NC group, #p < .05.
Figure 5. HNF4α is a potential target gene of miR-34a. (A) The binding site of HNF4α to miR-34a; (B) luciferase activity assay. Note: Compared with the miR-NC group, *p < .05.
Figure 6. Overexpression of HNF4 can reverse the inhibitory effect of miR-34a on proliferation, migration and invasion of SH-SY5Y cells. (A) Proliferation detection of SH-SY5Y cells in each group; (B) migration and invasion detection of SH-SY5Y cells in each group. Note: Compared with the miR-NC group, *p < .05; compared with miR-34a mimics + pcDNA3.1 group, #p < .05.
