Figures & data
Figure 1. Expression of miR-34c-5p and FLOT2 in osteosarcoma tissues and cells. (A) The expression of miR-34c-5p in osteosarcoma tissues and adjacent normal tissues. (B) The expression of FLOT2 mRNA in osteosarcoma tissues and adjacent normal tissues. (C, D) The expression of FLOT2 protein in osteosarcoma tissues and adjacent normal tissues. (E) Expression of miR-34c-5p in osteosarcoma cells and normal osteoblasts. (F) Expression of FLOT2 mRNA in osteosarcoma cells and normal osteoblasts. (G, H) Expression of FLOT2 protein in osteosarcoma cells and normal osteoblasts. Note: Compared with hFOB1.19 group, #p < .01, *p < .05 (B–D).
Figure 2. Effect of miR-34c-5p overexpression on proliferation of osteosarcoma cells. (A) Relative expression of miR-34c-5p. (B, C) Detection of osteosarcoma cell viability. (D–F) Expression of cell proliferation-related proteins. Note: Compared with miR-NC group, *p < .05.
Figure 3. Effect of miR-34c-5p overexpression on migration and invasion of osteosarcoma cells. (A–D) The migration and invasion of osteosarcoma cells. (E–G) Expression of cell migration and invasion related proteins. Note: Compared with miR-NC group, *p < .05.
Figure 4. Effect of inhibition of FLOT2 expression on proliferation, migration and invasion of osteosarcoma cells. (A) The migration and invasion of osteosarcoma cells. (B, C) Relative expression of FLOT2 protein. (D, E) Effect of inhibiting FLOT2 expression on proliferation of osteosarcoma cells. (F, G) Inhibition of FLOT2 expression on migration and invasion of osteosarcoma cells. (H–J) Effect of inhibition of FLOT2 expression on proliferation-related protein expression in osteosarcoma cells. (K–M) Effect of inhibition of FLOT2 expression on expression of migration-invasive-related proteins in osteosarcoma cells. Note: Compared with si-NC group, *p < .05
Figure 5. miR-34c-5p targeted regulation of FLOT2 expression. (A, D) The 3'UTR of FLOT2 contains a nucleotide sequence complementary to miR-34c-5p. (B, C, E, F) Dual luciferase reporter experiment. (G, H) miR-34c-5p regulates the expression of FLOT2 protein. Note: Compared with miR-NC group, *p < .05; Compared with anti-miR-NC group, #p < .05
Figure 6. FLOT2 overexpression reverses the effect of miR-34c-5p overexpression on proliferation, migration and invasion of osteosarcoma cells. (A) The migration and invasion of osteosarcoma cells. (B, C) Relative expression of FLOT2 protein. (D, E) FLOT2 overexpression reverses the effect of miR-34c-5p overexpression on proliferation of MG63 and SAOS-2 cells. (F, G) FLOT2 overexpression reverses the effect of miR-34c-5p overexpression on migration and invasion of MG63 and SAOS-2 cells. (H–J) FLOT2 overexpression reverses the effect of miR-34c-5p overexpression on the expression of proliferation-related proteins in MG63 and SAOS-2 cells. (K–M) FLOT2 overexpression reverses the effect of miR-34c-5p overexpression on the expression of migration-invasive proteins in MG63 and SAOS-2 cells. Note: Compared with miR-NC group,*p < .05; Compared with miR-34c-5p + pcDNA group, #p < .05
