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Research Article

Synthesis and characterization of gold nanoparticles from Marsdenia tenacissima and its anticancer activity of liver cancer HepG2 cells

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Pages 3029-3036 | Received 18 May 2019, Accepted 07 Jul 2019, Published online: 22 Jul 2019

Figures & data

Figure 1. UV-visible spectrum absorption pattern of AuNPs synthesized from MT.

Figure 1. UV-visible spectrum absorption pattern of AuNPs synthesized from MT.

Figure 2. HR-TEM analysis and XRD pattern of AuNPs synthesized from MT.

Figure 2. HR-TEM analysis and XRD pattern of AuNPs synthesized from MT.

Figure 3. FTIR analysis of AuNPs synthesized from MT.

Figure 3. FTIR analysis of AuNPs synthesized from MT.

Figure 4. MT-AuNPs inhibit the viability of HepG2 liver carcinoma cells. This experiment was repeated thrice and the bars in the graph represent S.E. (*p<.05).

Figure 4. MT-AuNPs inhibit the viability of HepG2 liver carcinoma cells. This experiment was repeated thrice and the bars in the graph represent S.E. (*p<.05).

Figure 5. MT-AuNPs inhibit the cell migration of HepG2 liver carcinoma cells.

Figure 5. MT-AuNPs inhibit the cell migration of HepG2 liver carcinoma cells.

Figure 6. MT-AuNPs induce intracellular ROS generation in HepG2 liver cancer cells.

Figure 6. MT-AuNPs induce intracellular ROS generation in HepG2 liver cancer cells.

Figure 7. MT-AuNPs changes the MMP in HepG2 liver cancer cells.

Figure 7. MT-AuNPs changes the MMP in HepG2 liver cancer cells.

Figure 8. Effect of MT-AuNPs on colorimetric caspase activity in HepG2 liver cancer cells by ELISA method. This experiment was repeated thrice and the bars in the graph represent S.E. (*p<.05, #p<.01).

Figure 8. Effect of MT-AuNPs on colorimetric caspase activity in HepG2 liver cancer cells by ELISA method. This experiment was repeated thrice and the bars in the graph represent S.E. (*p<.05, #p<.01).

Figure 9. The anticancer effect of MT-AuNPs on apoptotic signaling proteins in HepG2 cell lines were examined by Western blotting technique. The cells were treated with MT-AuNPs (50 and 75 μg) for 24 h and the protein expressions of Bcl-2, Bax, Bcl-XL, caspase-3 and caspase-9 were determined. β-actin was used as a loading control.

Figure 9. The anticancer effect of MT-AuNPs on apoptotic signaling proteins in HepG2 cell lines were examined by Western blotting technique. The cells were treated with MT-AuNPs (50 and 75 μg) for 24 h and the protein expressions of Bcl-2, Bax, Bcl-XL, caspase-3 and caspase-9 were determined. β-actin was used as a loading control.