The diagnostic effect of sequential detection of ADA screening and T-SPOT assay in pleural effusion patients

Figures & data

Table 1. Baseline characteristics of patients.

	Pleural tuberculosis (n = 172)	Non-pleural tuberculosis (n = 76)	p Value
Age, years (SE, range)	43.5 (1.35, 13–85)	66.50 (3.50, 45–87)	<.001
Sex, male (%)	127 (73.83%)	41 (53.94%)	.003
T-SPOT positive (%)	170 (98.83%)	31 (40.79%)	<.001
T-SPOT. A	25.44 (1.52, 22.44–28.44)	9.17 (1.86, 5.46–12.88)	<.001
T-SPOT. B	29.88 (1.97, 25.99–33.76)	9.03 (2.05, 4.95–12.10)	<.001
Effusion location
Right predominant	83 (48.26%)	39 (51.32%)	.681
Bilateral	56 (32.56%)	26 (34.21%)	.884
Effusion amount			.981
Small	18 (10.46%)	8 (10.53%)
Moderate	95 (55.23%)	41 (53.95%)
Massive	59 (34.31%)	27 (35.52%)
Serum ADA (IU/L)	12.68 (0.54, 11.59–13.76)	11.70 (0.86, 9.84–13.57)	.20
Serum LDH (IU/L)	182.29 (9.41, 163.37–201.20)	207.66 (11.88, 181.99–233.33)	.57
Pleural ADA (IU/L)	30.45 (6.73, 17.14–43.74)	10.73 (1.39, 7.73–13.72)	<.001
ADA (≧40 IU/L) (%)	8 (4.65%)	1 (1.31%)
ADA (<40 IU/L) (%)	164 (95.35%)	75 (98.69%)
Pleural LDH (IU/L)	549.46 (120.45, 307.27–791.65)	487.34 (134.51, 196.75–777.94)	.035
ESR (mm/h)	57.88 (4.80, 48.23–67.52)	51.43 (9.15, 31.67–71.19)	.63

The data are presented as n (%) or the mean [(SD; range) or (SE, 95%CI)]. T-SPOT TB: interferon-gamma release assay (IGRA), T-STOT. TB™; ADA: adenosine deaminase; LDH: lactate dehydrogenase; ESR: erythrocyte sedimentation rate.

Figure 1. The comparison of pleural ADA levels between TB group and non-TB group. ***: p < .001.

Figure 2. The positive rate of T-SPOT. A and B in TB and non-TB groups. ***: p < .001.

Table 2. The positive rate of T-SPOT.

	TB pleurisy group (n = 164)	Positive rate	Non-TB pleurisy group (n = 75)	Positive rate	p Value
T-SPOT. A (positive)	129	78.6%	9	12%	<.001
T-SPOT. B (positive)	134	81.7%	11	14.7%	<.001

A and B in TB and non-TB pleurisy groups.

Table 3. Logistic regression analysis.

Variable	Univariate		Multivariate
	OR (95% CI)	p Value	OR (95% CI)	p Value
Pleural ADA	0.92 (0.88–0.97)	.001	0.940 (0.901–0.981)	.005
T SPOT. A	0.924 (0.901–0.948)	.000	0.967 (0.943–0.991)	.008
T SPOT. B	0.938 (0.919–0.958)	.000	0.962 (0.933–0.991)	.011

Figure 3. ROC analysis based on the number of T-SPOT A blots in patients with pleurisy ADA levels below 40 IU/L. The area under the curve (AUC) was 0.822, with the sensitivity of 91.0% and specificity of 72.7%.

Figure 4. ROC analysis based on the number of T-SPOT B blots in patients with pleurisy ADA levels below 40 IU/L. The area under the curve (AUC) was 0.870, with the sensitivity of 95.5% and specificity of 77.3%.