Figures & data
Figure 1. Knockdown of circZFR inhibited the proliferation, migration and invasion of NSCLC cells. (A) Expression levels of circZFR in NSCLC tissues and adjacent normal tissues. *p < .05 vs. adjacent normal lung tissues. (B) Expression levels of circZFR in HBE cell line and NSCLC cell lines. *p < .05 vs. HBE cell line. (C, D) Transfection efficiency was confirmed using qRT-PCR after transfection with si-circZFR or si-NC in A549 and H1299 cells. (E, F) Cell proliferation of A549 and H1299 cells was measured using CCK-8 assay. (G, H) Cell migration and invasion of A549 and H1299 cells were assessed using the Transwell assay. *p < .05 vs. cells transfected with si-NC.
Figure 2. CircZFR regulated CUL4B expression by directly targeting miR-101-3p. (A) The putative sequences of miR-101-3p and circZFR. (C) The putative sequences of miR-101-3p and the 3’-UTR of CUL4B mRNA. (B, D) Luciferase reporter assay was performed to validate these predictions. *p < .05 vs. NC group. (E) Expression levels of miR-101-3p after transfection with si-circZFR or si-NC. *p < .05 vs. si-NC group. (F) Expression levels of CUL4B after transfection with miR-101-3p mimics or control mimics. *p < .05 vs. negative control (NC) group.
Figure 3. MiR-101-3p inhibitor prevented the effects of circZFR knockdown on NSCLC cells. (A) Expression levels of miR-101-3p in human NSCLC cell lines. *p < .05. (B) Transfection efficiency was confirmed after transfection with miR-101-3p inhibitor or control inhibitor in si-circZFR transfected A549 and H1299 cells. *p < .05 vs. si-NC + inhibitor-control; #p < .05 vs. si-circZFR + inhibitor-control. (C, D) Cell proliferation of A549 and H1299 cells was measured using CCK-8 assay. (E, F) Cell migration and invasion of A549 and H1299 cells was assessed using the Transwell assay. *p < .05 vs. si-NC + inhibitor-control; #p < .05 vs. si-circZFR + inhibitor-control.
Figure 4. CUL4B overexpression abolished the effects of miR-101-3p on NSCLC cells. (A–D) Protein expression levels of CUL4B after transfection with CUL4B-overexpressing plasmid in miR-101-3p transfected A549 and H1299 cells. (E–H) Cell proliferation, migration and invasion of A549 and H1299 cells were measured using CCK-8 assay and Transwell assay, respectively. *p < .05 vs. control; #p < .05 vs. miR-101-3p + pcDNA3.1.
