Figures & data
Figure 1. The survival curve of HNE-1 cells after treatment with different concentrations of Junduqing extractive which was determined by CCK-8 assay. The IC50 of Junduqing extractive in HNE-1 cells was 2.99 mg/ml.
![Figure 1. The survival curve of HNE-1 cells after treatment with different concentrations of Junduqing extractive which was determined by CCK-8 assay. The IC50 of Junduqing extractive in HNE-1 cells was 2.99 mg/ml.](/cms/asset/0e02c294-adca-4c29-b5b1-d0e7f24634c8/ianb_a_1667815_f0001_b.jpg)
Figure 2. The viability of HNE-1, HNE-2 and HONE1 cells in various groups which was examined by CCK-8 assay. Cells were cultured in the medium containing 1.0, 2.0 and 3.0 mg/ml of Junduqing extractive for various times. *p < .05 vs. Control; #p < .05 vs. 2.0 mg/ml.
![Figure 2. The viability of HNE-1, HNE-2 and HONE1 cells in various groups which was examined by CCK-8 assay. Cells were cultured in the medium containing 1.0, 2.0 and 3.0 mg/ml of Junduqing extractive for various times. *p < .05 vs. Control; #p < .05 vs. 2.0 mg/ml.](/cms/asset/431cbfdb-8588-489f-a572-1438996e52af/ianb_a_1667815_f0002_b.jpg)
Figure 3. The apoptosis of HNE-1, HNE-2 and HONE1 cells in various groups which was assessed by Annexin V-FITC/PI staining. Cells were cultured in the medium containing 1.0, 2.0 and 3.0 mg/ml of Junduqing extractive for 24 h. *p < .05 vs. Control; #p < .05 vs. 2.0 mg/ml.
![Figure 3. The apoptosis of HNE-1, HNE-2 and HONE1 cells in various groups which was assessed by Annexin V-FITC/PI staining. Cells were cultured in the medium containing 1.0, 2.0 and 3.0 mg/ml of Junduqing extractive for 24 h. *p < .05 vs. Control; #p < .05 vs. 2.0 mg/ml.](/cms/asset/5c5d1b12-fbdb-4e5c-8457-7c5bd4fe7ac8/ianb_a_1667815_f0003_c.jpg)
Figure 4. The migration of HNE-1, HNE-2 and HONE1 cells in various groups which was evaluated by scratch wound assay. Cells were cultured in the medium containing 1.0, 2.0 and 3.0 mg/ml of Junduqing extractive for 24 h. *p < .05 vs. Control; #p < .05 vs. 2.0 mg/ml.
![Figure 4. The migration of HNE-1, HNE-2 and HONE1 cells in various groups which was evaluated by scratch wound assay. Cells were cultured in the medium containing 1.0, 2.0 and 3.0 mg/ml of Junduqing extractive for 24 h. *p < .05 vs. Control; #p < .05 vs. 2.0 mg/ml.](/cms/asset/6567ee5d-3188-47f6-904d-cb573d02b723/ianb_a_1667815_f0004_c.jpg)
Figure 5. The invasion of HNE-1, HNE-2 and HONE1 cells in various groups which was evaluated by Transwell assay. Cells were cultured in the medium containing 1.0, 2.0 and 3.0 mg/ml of Junduqing extractive for 24 h. *p < .05 vs. Control.
![Figure 5. The invasion of HNE-1, HNE-2 and HONE1 cells in various groups which was evaluated by Transwell assay. Cells were cultured in the medium containing 1.0, 2.0 and 3.0 mg/ml of Junduqing extractive for 24 h. *p < .05 vs. Control.](/cms/asset/edb1c417-7968-4014-985e-fb4df89ef9f1/ianb_a_1667815_f0005_c.jpg)
Figure 6. The levels of Bcl-xL, Mcl-1, Caspase-3, Caspase-8 and Caspase-9 in HNE-1, HNE-2 and HONE1 cells of various groups which were examined by western blotting. Cells were cultured in the medium containing 1.0, 2.0 and 3.0 mg/ml of Junduqing extractive for 24 h. *p < .05 vs. Control; #p < .05 vs. 2.0 mg/ml.
![Figure 6. The levels of Bcl-xL, Mcl-1, Caspase-3, Caspase-8 and Caspase-9 in HNE-1, HNE-2 and HONE1 cells of various groups which were examined by western blotting. Cells were cultured in the medium containing 1.0, 2.0 and 3.0 mg/ml of Junduqing extractive for 24 h. *p < .05 vs. Control; #p < .05 vs. 2.0 mg/ml.](/cms/asset/891e2adb-8749-4e0c-9a24-e89a16f0f1b9/ianb_a_1667815_f0006_b.jpg)
Data availability
The analysed data sets generated during this study are available from the corresponding author on reasonable request.