Protective functions of salvianolic acid B in PC-12 cells against hydrogen peroxide-triggered damage by mediation of microRNA-26a

Figures & data

Figure 1. H₂O₂ caused PC-12 cell injury. (a) Roles of H₂O₂ in cell viability by CCK-8 assay. (b) Roles of H₂O₂ in cell migration by Transwell assay. (c) Roles of H₂O₂ in cell apoptosis by flow cytometry assay. (d) Roles of H₂O₂ in Bcl-2, Bax and pro/cleaved-caspase-3/-9 by Western blot assay. Results were acquired from three independent experiments and were emerged as the means ± SD. *p<.05, **p<.01, ***p<.001.

Figure 2. Sal-B protected PC-12 cells resisting H₂O₂-triggered injury. (a,b) Roles of Sal-B in cell viability by CCK-8 assay. (c) Roles of Sal-B in cell migration by Transwell assay. (d) Roles of Sal-B in cell apoptosis by flow cytometry assay. (e) Roles of Sal-B in Cyclin A, Cyclin E1, Cyclin D1, CDK2 and CDK4 by Western blot assay. (f) Roles of Sal-B in Bcl-2, Bax and pro/cleaved-caspase-3/-9 by Western blot assay. Results were acquired from three independent experiments and were emerged as the means ± SD. *p < .05, **p < .01, ***p < .001.

Figure 3. Sal-B elevated miR-26a expression in H₂O₂-corrupted PC-12 cells. Roles of Sal-B in miR-26a expression in H₂O₂-corrupted PC-12 cells by qRT-PCR assay. Results were acquired from three independent experiments and were emerged as the means ± SD. *p < .05.

Figure 4. Sal-B protected PC-12 cells resisting H₂O₂-triggered injury by elevation of miR-26a. (a) The expression of miR-26a in miR-26a inhibitor and NC-transfected PC-12 cells. (b) Roles of miR-26a inhibition in cell viability in Sal-B and H₂O₂ co-managed PC-12 cells by CCK-8 assay. (c) Roles of miR-26a inhibition in cell migration in Sal-B and H₂O₂ co-managed PC-12 cells by Transwell assay. (d) Roles of miR-26a inhibition in cell apoptosis in Sal-B and H₂O₂ co-managed PC-12 cells by flow cytometry assay. (e) Roles of miR-26a inhibition in Cyclin A, Cyclin E1, Cyclin D1, CDK2 and CDK4 in Sal-B and H₂O₂ co-managed PC-12 cells by Western blot assay. (f) Roles of miR-26a inhibition in Bax, Bcl-2 and pro/cleaved-caspase-3/-9 in Sal-B and H₂O₂ co-managed PC-12 cells by Western blot assay. Results were acquired from three independent experiments and were emerged as the means ± SD. *p < .05, **p < .01, ***p < .001.

Figure 5. Sal-B evoked the activations of PI3K/AKT and MEK/ERK pathways by enhancing miR-26a. (a) Roles of Sal-B and miR-26a inhibition in PI3K/AKT pathway in H₂O₂-managed PC-12 cells by Western blot assay. (b) Roles of Sal-B and miR-26a inhibition in MEK/ERK pathway in H₂O₂-managed PC-12 cells by Western blot assay. Results were acquired from three independent experiments and were emerged as the means ± SD. ***p < .001.