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Emerging Microbes & Infections Volume 9, 2020 - Issue 1
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Articles

Precise diagnosis of Neisseria macacae infective endocarditis assisted by nanopore sequencing

Jing-Wen Aia Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, People’s Republic of ChinaView further author information
,
Hong Liub Department of Clinical Laboratory, Huashan Hospital, Fudan University, Shanghai, People’s Republic of ChinaView further author information
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Hui-Xia Lia Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China;c Department of Infectious Diseases, No. 988. Hospital of Liberation Army, Henan, People’s Republic of ChinaView further author information
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Qing-Xia Linga Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China;d Department of Infectious Diseases, Qingpu Branch of Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of ChinaView further author information
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Yan-Qin Aia Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China;e Department of Infectious Diseases, Xuchang Central Hospital affiliated to Henan University of Science and Technology, Henan, People’s Republic of ChinaView further author information
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Si-Jia Suna Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China;f Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai, People’s Republic of ChinaView further author information
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Xuan Wanga Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, People’s Republic of ChinaView further author information
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Bing-Yan Zhanga Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, People’s Republic of ChinaView further author information
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Jian-Ming Zhenga Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, People’s Republic of ChinaCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0003-1471-9078View further author information
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Jia-Lin Jina Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, People’s Republic of ChinaCorrespondence[email protected]
View further author information
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Wen-Hong Zhanga Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, People’s Republic of ChinaCorrespondence[email protected]
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Pages 1864-1868 | Received 10 Mar 2020, Accepted 05 Aug 2020, Published online: 24 Aug 2020

Figures & data

Figure 1. The blood culture was positive for N. macacae in 24 h. (A) Bacterial colony smear (Gram staining). (B) Blood culture was positive, then direct smear (Gram staining). (C) Blood plate for 72 h. (D) Chocolate plate for 72 h. (E) Time curve of blood bottle positive report. The blood culture was positive at 23 h 6 min. (F) The strain from blood culture was identified using MALDITOF MS.

Figure 1. The blood culture was positive for N. macacae in 24 h. (A) Bacterial colony smear (Gram staining). (B) Blood culture was positive, then direct smear (Gram staining). (C) Blood plate for 72 h. (D) Chocolate plate for 72 h. (E) Time curve of blood bottle positive report. The blood culture was positive at 23 h 6 min. (F) The strain from blood culture was identified using MALDITOF MS.

Figure 2. The results of 16S RNA sequencing. It was 99.93% of similarity with the genbank sequence reference: N. macacae strain M-740 16S ribosomal RNA part sequence.

Figure 2. The results of 16S RNA sequencing. It was 99.93% of similarity with the genbank sequence reference: N. macacae strain M-740 16S ribosomal RNA part sequence.

Figure 3. The results of vegetation detection. (A) Vegetations in sterile tubes, about 10 × 5 mm. (B) Sequencing analysis in 1 h. (C) Sequencing analysis in 4 h. (D) Sequencing analysis in 12 h.

Figure 3. The results of vegetation detection. (A) Vegetations in sterile tubes, about 10 × 5 mm. (B) Sequencing analysis in 1 h. (C) Sequencing analysis in 4 h. (D) Sequencing analysis in 12 h.

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