Toward a better understanding of enteric gliogenesis

Figures & data

Figure 1. Key cell-specific markers during ENS formation. Enteric NCCs of vagal origin (ENS progenitors) enter the foregut around E9.5 and start to migrate rostrocaudally. Soon after their arrival, a subset of these ENS progenitors starts to differentiate into neurons while the majority is maintained in an undifferentiated and proliferative state. The competency of a subset of ENS progenitors to differentiate into enteric glial cells is only acquired around E11.5. From E15.5 onwards, colonization of the gut is completed but neuronal and glial differentiation continue until birth and during a short postnatal period. Adapted from refs. 20, 21.

Table 1. Extract of Spot^Tg/Tg vs control RNAseq data from e12.5 enteric NCCs.³⁹

		FPKM
	Transcript isoform	Spot^Tg/Tg	Ctl	Fold Change	Significant(P ≤ 0.01)
Nr2f1 locus	A830082K12Rik-001	9.10	2.07	+4.4	yes
	Nr2f1–001	94.92	7.07	+13.4	yes
Selected glial markers	Cnp-001	48.96	31.36	+1.6	yes
	Erbb3–001	81.34	46.32	+1.8	yes
	Fabp7–001	190.92	62.34	+3.1	yes
	Foxd3–001	55.92	33.54	+1.8	yes
	Mbp-003	3.47	5.24	−1.4	yes
	Mpz-001	3.56	0.95	+3.9	yes
	Nr2f2–001	7.31	6.25	+1.2	no
	Plp1–002	25.74	14.79	+1.8	yes
	Pmp22–001	4.24	4.66	−1.1	no
	Pou3f1–001	0.67	0.18	+3.7	yes
	S100b-001	3.20	0.56	+5.6	yes
	Sox2–001	27.91	21.66	+1.3	yes
	Sox8–001	17.27	23.69	−1.4	yes
	Sox10–201	64.41	36.05	+1.8	yes
	Zeb2–002	22.87	17.49	+1.3	no
Selected components of the Notch pathway	Dll1–001	7.36	10.44	−1.4	yes
	Dll3–001	7.62	7.49	−	no
	Dll4–001	0.59	1.33	−2.2	yes
	Hes1–001	14.89	25.72	−1.7	yes
	Hes5–001	0.11	0.81	−7.1	yes
	Hes6–001	29.32	36.63	−1.2	no
	Hey1–001	4.04	6.16	−1.5	yes
	Hey2–001	3,70	3,45	+1.1	no
	Jag1–001	5,67	3,08	+1.8	yes
	Jag2–201	1,23	2,86	−2.2	yes
	Notch1–001	15,49	10,63	+1.5	yes
	Notch2–001	13.95	17.42	−1.2	yes
	Notch3–001	6.24	5.16	+1.2	yes
	Notch4–001	0.28	0.41	+1.6	no
Selected components of the Hedgehog pathway	Gas1–001	8.03	10.19	−1.3	yes
	Gli1–201	1.08	2.31	−2.1	yes
	Gli2–001	1.23	1.70	−1.4	yes
	Gli3–001	7.86	5.93	+1.3	yes
	Ptch1–001	5.92	9.22	−1.5	yes
	Ptch2–001	0.47	0.90	−1.9	yes
	Smo-001	37.18	27.86	+1.4	yes
	Sufu-001	4.01	3.91	−	no

Note. FPKM, fragments per kilobase of transcript per million mapped reads.

Bergeron KF, Nguyen CM, Cardinal T, Charrier B, Silversides DW, Pilon N. Upregulation of the Nr2f1-A830082K12Rik gene pair in murine neural crest cells results in a complex phenotype reminiscent of waardenburg syndrome type 4. Dis Model Mech 2016; 9:1283-93; https://doi.org/10.1242/dmm.026773

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