Presence of postprandial antidysmetabolic and antioxidative stress properties in aqueous methanol extract of seeds and tuber of aquatic food plant Nymphaea nouchali (Burm.f.)

Figures & data

Figure 1. Total polyphenol (TPP) and flavonoids (TF) values in aqueous methanol extract of N. nouchali.

Notes: The polyphenol content represent mg/mL gallic acid equivalent and total flavonoids were estimated in terms of mg/mL rutin equivalent concentration. Statistical values *p < 0.0029 and **p < 0.057. Unpaired t test with Welch’s correction was applied to find degree of significance. Values represent mean ± SE (n = at least three readings).

Figure 2. Rat intesitnal α-glucosidase inhibition by aqueous methanol extract of seeds and tuber of N. nouchali.

Notes: Activity was recorded with 2 mg/mL stock solution of the extract. One way ANOVA followed by Tukey’s Multiple Comparison Test was applyied to calculate differences within the groups. ^ap < 0.01, ^bp < 0.001, ^cp < 0.01. Values represent mean ± SD (n = 3).

Figure 3. Porcine pancreatic lipase inhibitory activity by aqueous methanol extract of seeds and tuber of N. nouchali.

Notes: Activity was recorded with 2 mg/mL stock solution of the extract. One way ANOVA followed by Tukey’s Multiple Comparison Test was applyied to calculate differences within the groups. ^ap = ns, ^bp = ns, ^cp < 0.05. Values represent mean ± SD (n = 3). ns (not significant).

Table 1. In-vitro free radicals scavenging, antioxidant activities in aqueous methanol extract of seeds and tuber of N. nouchali

Samples	ABTS % inhibition (SC50)	DPPH % inhibition (SC50)	NBT % inhibition (RP50)	FeCl3 % inhibition (RP50)	AGEs % inhibition
					Vesperlysine-type	Pentosidine-type
Tuber	99.4 ± 0.01 (6.4)	85.2 ± 0.6 (26.1)	99.4 ± 0.05 (8.69)	94.16 ± 0.01 (0.02)	34.78 ± 0.66	97.37 ± 0.02
Seeds	96.44 ± 0.05 (6.3)	83.5 ± 2.20 (13.8)	99.7 ± 0.03 (9.79)	95.69 ± 0.00 (0.01)	87.94 ± 1.05	89.08 ± 0.51
Ascorbic acid	99.56 ± 0.00 (3.88)	99.70 ± 0.02 (11.98)	99.8 ± 0.03 (4.62)	95.71 ± 0.20 (0.03)	–	–
Aminoguanidine	–	–	–	–	74.53 ± 0.30	90.02 ± 8.07

Notes: % inhibition recorded with 2 mg/mL stock solution of the 50% aqueous methanol solution of Nymphaea nouchali seeds and tuber except for NBT and FeCl₃ where 1 mg/mL stock solution was used. The quantity of test solution reacted with respective test reagents are mentioned in material and method section. The units for radicals scavenging (SC₅₀) and reducing power (RP₅₀) 50% concentrations are presented in parentheses (μg in reaction mixture). Values represent mean ± SD of at least three experiments.