Anti-inflammatory activity of Juniper (Juniperus communis) berry essential oil in human dermal fibroblasts

Figures & data

Figure 1. The bioactivity profile of juniper berry essential oil (JEO, 0.003%, v/v in DMSO) using the BioMAP System HDF3CGF.

Notes: The x-axis denotes the protein-based biomarker readouts. The y-axis denotes the relative expression levels of biomarkers compared to vehicle control values, in log form. Vehicle control values are shaded in gray, denoting the 95% confidence level. The asterisks (*) indicate the biomarkers designated with “key activity,” i.e., the biomarker values were significantly different (p < 0.05) after cell treatment with 0.003% JEO, compared to those of vehicle controls, outside of the significance envelope, with an effect size of at least 10% (more than 0.05 log ratio units). DMSO, dimethyl sulfoxide; MCP-1, monocyte chemoattractant protein; VCAM-1, vascular cell adhesion molecule 1; ICAM-1, intracellular cell adhesion molecule 1; IP-10, interferon gamma-induced protein 10; I-TAC, interferon-inducible T-cell alpha chemoattractant; IL-8, interleukin-8; MIG, monokine induced by gamma interferon; EGFR, epidermal growth factor receptor; M-CSF, macrophage colony-stimulating factor; MMP-1, matrix metalloproteinase 1; PAI-1, plasminogen activator inhibitor 1; TIMP, tissue inhibitor of metalloproteinase.

Figure 2. The top 20 canonical pathways matching the gene expression bioactivity profile of juniper berry essential oil (JEO) in the HDF3CGF system, produced via Ingenuity Pathway Analysis (Qiagen, www.qiagen.com/ingenuity).

Notes: Each p-value was calculated with the right-tailed Fisher’s Exact Test. The p-value measures the likelihood that the observed association between a specific pathway and the data-set is due to random chance. The smaller the p value (the bigger the - ln (p-value), indicated by the black bars) that the pathway has, the more significantly it matches with the bioactivity of JEO. The ratio, indicated by each gray bar, was calculated by taking the number of genes from the JEO data-set that participate in a canonical pathway and dividing it by the total number of genes in that pathway. JAK2, Janus kinase 2.