GRASP55 facilitates autophagosome maturation under glucose deprivation

Figures & data

Figure 1. Dual function of GRASP55 in the organization of intracellular membranes. Under growth condition, GRASP55 is O-GlcNAcylated and localized in the Golgi, where it serves as the “glue” to hold adjacent Golgi cisternae into stacks by forming trans-oligomers. Upon glucose starvation, GRASP55 is de-O-GlcNAcylated and targeted to the autophagosome-lysosome interface, where it interacts with LC3-II and LAMP2 and functions as a membrane tether to facilitate autophagosome-lysosome fusion.