Genetic identification of marine eels (Anguilliformes: Congroidei) through DNA barcoding from Kasimedu fishing harbour

Figures & data

Figure 1. Collection of the sampling locality for the specimens collected in this study.

Table 1. List of species included in the study for COI sequence analysis and accession number and barcode index numbers from suborder Congroidei.

Family	Genus	Species	No. of samples	GenBank ID	BINs
Congridae	Ariosoma	Ariosoma meeki	2	MW311323- MW311324	BOLD:AAF6666
		Ariosoma shiroanago	1	MW311321	BOLD:ADC9223
		Ariosoma sp. 1	4	MW311322, MW311325- MW311327	BOLD:AEI5786
		Ariosoma sp. 2	3	MW311318- MW311320	BOLD:AEI5787
	Conger	Conger cinereus	3	MW306768- MW306770	BOLD:AAD1242
	Gnathophis	Gnathophis musteliceps	2	MW306762- MW306763	BOLD:AEJ2482
		Gnathophis sp. 1	1	MW306760	BOLD:AAG4489
		Gnathophis sp. 2	1	MW306761	BOLD:ABA5210
	Uroconger	Uroconger lepturus	10	MW351781- MW351785, MW310970- MW310973, MW387622	BOLD:AAD1243
					BOLD:AAJ8378
Muraenesocidae	Gavialiceps	Gavialiceps taiwanensis	3	MW351778- MW351780	BOLD:AEJ8964
					BOLD:AAF4895
	Muraenesox	Muraenesox bagio	1	MW306767	BOLD:ACK7558
		Muraenesox cinereus	3	MW306764- MW306766	BOLD:ACB5037
	Sauromuraenesox	Sauromuraenesox vorax	5	MW044564- MW044568	BOLD:AEI3835
Nettastomatidae	Facciolella	Facciolella oxyrhyncha	1	MW306758	BOLD:AAB6640
		Facciolella sp.	2	MW306756- MW306757	BOLD:AEJ3046
Ophichthidae	Ophichthus	Ophichthus chennaiensis	1	MW366902	BOLD:AEI1684
	Pisodonophis	Pisodonophis cancrivorus	1	MW306759	BOLD:AAW5582

Table 2. Genetic distance (% K2P) was observed within various taxonomic levels with NCBI data.

Comparison within	Taxa	Mean	Minimum	Maximum	Standard error
Species	17	3.08	0.00	13.10	0.14
Genus	10	6.80	0.82	14.38	0.67
Family	4	13.80	6.03	20.83	0.38

Table 3. Pair-wise genetic distance using the kimura 2-parameter model (K2P) between 17 species of marine eels from suborder Congroidei with NCBI haplotype.

	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16
1 Urocongerlepturus
2 Sauromuraenesoxvorax	0.2137
3 Gnathophis sp.1	0.2157	0.2400
4 Gnathophis sp. 2	0.2194	0.2425	0.1161
5 Gnathophismusteliceps	0.2360	0.2339	0.2024	0.1737
6 Gavialicepstaiwanensis	0.2143	0.2304	0.1951	0.2034	0.2122
7 Facciolella sp.	0.2132	0.2161	0.2309	0.2212	0.2361	0.2036
8 Facciolellaoxyrhyncha	0.2204	0.1960	0.2223	0.2031	0.2290	0.2215	0.1466
9 Ophichthuschennaiensis	0.2094	0.1902	0.1980	0.1958	0.2219	0.2223	0.2185	0.2017
10 Pisodonophiscancrivorus	0.2176	0.2149	0.2186	0.2038	0.2239	0.2079	0.2111	0.2036	0.1712
11 Ariosoma sp.1	0.2576	0.2573	0.2805	0.2771	0.2724	0.2779	0.2576	0.2657	0.2376	0.2687
12 Ariosomashiroanago	0.2324	0.2439	0.2503	0.2562	0.2670	0.2581	0.2211	0.2316	0.2130	0.2496	0.1019
13 Ariosomameeki	0.2452	0.2663	0.2713	0.2602	0.2676	0.2752	0.2513	0.2533	0.2415	0.2627	0.1685	0.1449
14 Ariosoma sp. 2	0.2378	0.2323	0.2654	0.2485	0.2605	0.2577	0.2257	0.2376	0.2082	0.2438	0.1600	0.1572	0.1892
15 Muraenesox cinereus	0.2407	0.2468	0.2174	0.2225	0.2574	0.2395	0.2701	0.2381	0.2251	0.2343	0.3070	0.2834	0.2728	0.2732
16 Muraenesoxbagio	0.2322	0.2199	0.2288	0.2244	0.2559	0.2334	0.2487	0.2297	0.2076	0.2286	0.2847	0.2679	0.2853	0.2673	0.1332
17 Conger cinereus	0.2218	0.2082	0.2112	0.2250	0.2645	0.2402	0.2446	0.2280	0.1998	0.2389	0.2901	0.2614	0.2664	0.2455	0.2623	0.2436

Figure 2. The Bayesian Inference (BI) analysis shows the multiple clades in marine eels of COI gene with NCBI database. Color specimens show the present study. Color branches indicate the separate group of U. lepturus. Values near branches show Bayesian posterior probability (PP).

Figure 3. COI gene species delimitation of suborder Congroidei by ASAP and BINs. Colors represent unique partitions.

Data availability statement

The data that support the findings of this study are original and openly available in NCBI at https://www.ncbi.nlm.nih.gov/.

Accession number: MW311323- MW311324, MW311321, MW311322, MW311325- MW311327, MW311318-MW311320, MW306768-MW306770, MW306762-MW306763, MW306760, MW306761, MW351781-MW351785, MW310970-MW310973, MW387622, MW351778-MW351780, MW306767, MW306764-MW306766, MW044564-MW044568, MW306758, MW306756-MW306757, MW366902, MW306759