Figures & data
Figure 1. After treatment of whole blood or plasma with organic solvent to precipitate proteins and then centrifugation, the supernatant is analyzed using reversed phase ultrahigh-pressure liquid chromatography-tandem mass-spectrometry (UHPLC-MS/MS). In this example, the long-acting anticoagulant rodenticides (LAARs) brodifacoum, bromadiolone, difenacoum, difethialone, and flocoumafen were extracted from 100 µL human plasma, separated using reversed phase UHPLC and measured in a single analysis using negative ion electrospray tandem mass spectrometry with selected-reaction monitoring (SRM). Note that each racemic LAAR was detected as a pair of cis/trans diastereomers due to the presence of two chiral centers.
