Effects of Enzymatic Treatment of Corn Gluten Meal on Lutein and Zeaxanthin Extraction

Figures & data

Table 1 Orthogonal experimental design in levels of variables of enzymatic treatment of CGM

Levels	A (Enzyme content (U/g protein))	B (Hydrolysis time (h))	C (Solid content of slurry (%))
1	107	1	5
2	160.5	2	10
3	214	3	15

Figure 1 Effect of enzymatic treatment on total carotenoids extraction.

Figure 2 Effect of enzymatic treatment on lutein and zeaxanthin extraction.

Figure 3 Effects of solid content in the slurries on total carotenoids extraction.

Figure 4 Effects of solid content in the slurries on lutein and zeaxanthin extraction.

Figure 5 Effects of hydrolysis time on total carotenoids extraction.

Figure 6 Effects of hydrolysis time on lutein and zeaxanthin extraction.

Table 2 The results of orthogonal experiments for enzymatic treatment of CGM (Yield of carotenoids: µg/g, CGM, on moisture-free basis)

Number of experiments	A	B	C	Yield of total carotenoids	Yield of crude lutein	Yield of crude zeaxanthin
1	107	1	5	537	63	65.6
2	107	2	10	527	82.4	96.1
3	107	3	15	523	77.9	115
4	160.5	1	10	512	78.9	113.1
5	160.5	2	15	558	82.6	125.8
6	160.5	3	5	532	67.3	107
7	214	1	15	550	87.7	111.6
8	214	2	5	553	92.3	129.5
9	214	3	10	520	88	123

Table 3 The statistically analyzed results of orthogonal experiments for enzymatic treatment of CGM

Yield of total carotenoids			Yield of crude lutein			Yield of crude zeaxanthin
A	B	C	A	B	C	A	B	C
K1	27	39	62.1	13.29	19.5	12.6	11.7	9.6	21.6
K2	42	78	0.99	18.9	47.31	39.3	15.9	21.39	2.49
K3	63	15	71.1	57.9	23.1	38.1	48.9	15.3	22.5
k1	9	13	20.7	4.43	6.5	4.2	−3.9	3.2	7.2
k2	14	26	0.33	6.3	15.77	13.1	5.3	7.13	0.83
k3	21	5	23.7	19.3	7.7	12.7	16.3	5.1	7.5
R	12	21	23.4	14.87	9.27	8.9	20.2	3.93	6.67