Effect of induced mild hypothermia on two pro-inflammatory cytokines and oxidative parameters during experimental acute sepsis

Figures & data

Figure 1. Schematic representation of experimental procedure.

Figure 2. Cytokines values (A, IL-6 and B, TNF-α) in plasma for each experimental condition (n = 6 for each group except for septic group in normothermia where n = 4). Data are expressed as median ± interquartile range. *Significant difference between septic and sham normothermia groups. ^#Significant difference between septic and sham hypothermia groups. ⁺Significant difference between septic normothermia and septic hypothermia groups (P < 0.05).

Figure 3. Nitric oxide (NO) level in plasma for each experimental condition (n = 6 for each group except for septic group in normothermia where n = 4 and for sham and septic groups in induced mild hypothermia where n = 5). *Significant difference between septic and sham normothermia groups (P < 0.05). ^xSignificant difference between sham normothermia and sham hypothermia groups (P < 0.05).

Figure 4. Hydroxyl radical (HO^•) production in plasma per hour for each experimental condition (n = 6 for each group except for sham and septic groups in normothermia where n = 5 and n = 4, respectively). Data are expressed as median ± interquartile range *Significant difference between septic and sham normothermia groups. ^#Significant difference between septic and sham hypothermia groups (P < 0.05).

Table 1. SOD and CAT activity and MDA concentrations in the heart for each experimental condition

	Normothermia			Hypothermia
	Baseline	Sham	Septic	Baseline	Sham	Septic
SOD (USOD/mg protein)	7 ± 1	6 ± 2	7 ± 1	6 ± 1	6 ± 1	6 ± 1
CAT (mmol H2O2/min/mg protein)	57 ± 20	67 ± 15	89 ± 22	60 ± 15	70 ± 13	61 ± 23
MDA (nmol/g de T)	43 ± 8	36 ± 1	35 ± 18	68 ± 14	42 ± 5	36 ± 9*

Data are reported as median ±interquartile range.

* P < 0.05. A significant difference between sham and septic rats.