Dietary Aronia melanocarpa extract enhances mTORC1 signaling, but has no effect on protein synthesis and protein breakdown-related signaling, in response to resistance exercise in rat skeletal muscle

Figures & data

Table 1 Components of AME

Fig. 1 Schematic of the experimental protocol

Table 2 Food consumption and body weight

Fig. 2 Phosphorylation of upstream substrates of mTORC1 relative to total protein content after resistance exercise. a Representative western blots. b Phosphorylated Akt at Ser473. c Phosphorylated ERK1/2 at Ser240/244. Values are means ± SEM. *P < 0.05, effect of diet; #P < 0.05, effect of exercise. AME, Aronia melanocarpa extract. EXE, exercise. A.U., arbitrary units

Fig. 3 Phosphorylation of markers of mTORC1 activity relative to total protein content after resistance exercise. a Representative western blots. b Phosphorylated mTOR at Ser2448. c Phosphorylated p70S6K at Thr389. d Phosphorylated rpS6 at Thr240/244. Values are means ± SEM. #P < 0.05, effect of exercise; †P < 0.05 vs. control leg in the same group; §P < 0.05 vs. corresponding leg in the NOR group. AME, Aronia melanocarpa extract. EXE, exercise. A.U., arbitrary units

Fig. 4 Muscle protein synthesis rate at 6 h after resistance exercise. a Representative western blots. b Quantification of muscle protein synthesis. Values are means ± SEM. # P < 0.05, significant main effect of exercise. A.U., arbitrary units. AME, Aronia melanocarpa extract, EXE, exercise

Fig. 5 Phosphorylation relative to total protein content and expression of autophagy-lysosome system mediators after exercise. a Representative western blots. b Phosphorylated AMPK at Thr172. c Phosphorylated ULK1 at Thr757. d Phosphorylated ULK1 at Ser317. e LC3B-II expression. f Ratio of LC3B-II to -I. g P62 expression. Values are means ± SEM. *P < 0.05, effect of diet; #P < 0.05, effect of exercise; ¶ trend for exercise. AME, Aronia melanocarpa extract. EXE, exercise. A.U., arbitrary units

Fig. 6 Expression of polyubiquitinated proteins and mRNA expression of mediators of UPS after resistance exercise. a Representative western blots. b MAFbx expression. c MuRF1 expression. d Polyubiquitinated protein expression. Values are means ± SEM. *P < 0.05, effect of diet; #P < 0.05, effect of exercise. AME, Aronia melanocarpa extract. EXE, exercise. A.U., arbitrary units

Availability of data and materials

All data generated or analyzed during this study are included in this published article.