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Abstract
The peptidome, which is the low-molecular-weight subset of the proteome, has attracted increasing attention in recent years. However, with the interference of high-abundance protein components in complex biological mixtures (e.g., serum), selective extraction of endogenous peptides is the first and most important step before analyzing the peptidome. A number of methods and technologies have now been developed for the selective enrichment, fractionation, quantitative analysis of the endogenous peptides and their application in the potential biomarker discovery. This review will cover the methods and technologies developed in recent years for the peptidome analysis on the selective extraction, multidimensional separation and quantitative analysis, as well as their application for clinical diagnosis and biomarker discovery. The future prospects of the peptidome are also discussed.
Financial & competing interests disclosure
This study was supported by the National Natural Science Foundation of China (Nos. 20675081, 20735004), the China State Key Basic Research Program Grant (2005CB522701, 2007CB914104), the China High Technology Research Program Grant (No. 2006AA02A309, 2008ZX10002–017) and the Knowledge Innovation program of CAS (KJCX2.YW.HO9, KSCX2.YW.079) and the Knowledge Innovation program of DICP (to Hanfa Zou).
The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
No writing assistance was utilized in the production of this manuscript.