CD103+ T Lymphocyte Count Linked to the Thickness of Invasion on Acral Melanoma without E-Cadherin Involvement

Figures & data

Figure 1 Result of E-cadherin immunohistochemical staining.

Notes: (A) E-cadherin Immunoexpression strong intensity (magnification 400X). (B) E-cadherin Immunoexpression medium intensity (magnification 400X). (C) E-cadherin Immunoexpression weak intensity (magnification 400X).

Figure 2 Result of CD103 immunohistochemical staining.

Notes: (A) High immunoexpression of CD103 (magnification 400X). (B) Low immunoexpression of CD103 (magnification 400X).

Table 1 Research Subject Characteristics

Variables	N = 40
Age (years)
Mean ± SD.	58.02±13.23
Sex
Male	23 (57.5%)
Female	17 (42.5%)
Thickness of invasion (mm)
Mean ± SD.	7.85±6.39
E-cadherin
Low	14 (35%)
High	26 (65%)
CD103
Low	19 (47.5%)
High	21 (52.5%)
Clark level
II–III	19 (47.5%)
IV–V	21 (52.5%)
Breslow thickness
T1	0
T2	2 (5%)
T3	18 (45%)
T4	20 (50%)
Location
Palm	5 (12.5%)
Pedis	33 (82.5%)
Subungual	2 (5%)
TIL
Brisk	27 (67.5%)
Non-brisk	13 (32.5%)
Ulceration status
Yes	21 (52.5%)
No	19 (47.5%)

Abbreviation: TIL, tumor-infiltrating lymphocytes.

Table 2 Association of Clinical Patient Characteristics with the CD103 Immunoexpression

Variables	Cases		P-value
	Low CD103 Immunoexpression (N=19)	High CD103 Immunoexpression (N=21)
Age (years)			0.092
Mean ± SD.	62.5±11.5	53.5±13.5
Sex			0.337
Male	10 (53.6%)	14 (66.6%)
Female	9 (47.4%)	7 (33.4%)
Breslow thickness			0.0001
T2	0	2 (9.5%)
T3	3 (15.7%)	15 (71.4%)
T4	16 (84.3%)	4 (19.1%)
Location			0.085
Palm	1 (5.3%)	4 (19.1%)
Pedis	16 (84.2%)	17 (80.9%)
Subungual	2 (10.5%)	0
TIL			0.233
Brisk	12 (63.2%)	15 (71.4%)
Non-brisk	7 (36.8%)	6 (28.6%)
Ulceration status			0.251
Yes	9 (47.4%)	12 (57.1%)
No	10 (52.6%)	9 (42.9%)

Abbreviation: TIL, tumor-infiltrating lymphocytes.

Table 3 Association of E-Cadherin Immunoexpression with the Thickness of Invasion in Acral Melanoma

Variable	Thickness of Invasion		Total	P-value
	≤4 mm N=20	>4 mmN=20
E-cadherin				0.4272
Low	6 (15%)	8 (20%)	14 (35%)
High	14 (35%)	12 (30%)	26 (65%)

Notes: The unpaired categorical comparative hypothesis is calculated based on the chi-square test. The significance value was based on a p-value <0.05.

Table 4 Association of CD103 Immunoexpression with the Thickness of Invasion in Acral Melanoma

Variable	Thickness of Invasion		Total	P-value
	≤4 mm N=20	>4 mm N=20
CD103				0.0001*
Low	3 (7.5%)	16 (40%)	19 (47.5%)
High	17 (42.5%)	4 (10%)	21 (52.5%)

Notes: The unpaired categorical comparative hypothesis is calculated based on the Chi-square test. The significance value was based on a p-value <0.05. Sign *Indicates p-value <0.05, which means significant or statistically significant.