Higher susceptibility of NOD/LtSz-scid Il2rg^−/− NSG mice to xenotransplanted lung cancer cell lines

Figures & data

Figure 1 Tumorigenicity in mice after subcutaneous inoculation of EBC1 cells.

Notes: Tumorigenicity after inoculation of 10⁵ (A) and 10⁴ (B) EBC1 cells. (C) Tumorigenicity in NSG mice after inoculation of 10–10³ EBC1 cells. X-axis shows period after inoculation of cells (weeks). Y-axis shows mice with tumor formation (%). (D) Tumor sizes in NSG mice after inoculation of 10³ EBC1 cells. Each symbol represents an individual mouse. (E) At 12 weeks after inoculation of 10³ EBC1 cells, mice were euthanized. NOD-scid and NSG mice are shown.

Abbreviations: NSG, NOD/LtSz-scid Il2rg^−/−; NOD, non-obese diabetic.

Figure 2 Tumorigenicity in mice after subcutaneous inoculation of A549 cells.

Notes: Tumorigenicity after inoculation of 10⁵ (A) and 10⁴ (B) A549 cells. (C) Tumorigenicity in NSG mice after inoculation of 10–10³ A549 cells.

Abbreviation: NSG, NOD/LtSz-scid Il2rg^−/−.

Figure 3 Pathology of developed tumor after inoculation of 10 A549 cells.

Notes: (A) Hematoxylin and eosin staining. (B) Immunostaining for anti-human pancytokeratin. (C) Immunostaining for anti-human vimentin. (D) Negative control without primary antibodies.