Figures & data
Table 1 Characteristics of study subjects
Figure 1 Decreased expression of MBD2 in patients with COPD.
Abbreviations: ANOVA, analysis of variance; COPD, chronic obstructive pulmonary disease; MBD2, methyl-CpG-binding domain protein 2; IgG Ab, immunoglobulin G antibody.
![Figure 1 Decreased expression of MBD2 in patients with COPD.](/cms/asset/577782d4-5723-4cac-848f-3b062e3613e0/dcop_a_148595_f0001_c.jpg)
Figure 2 Lung function and histological measurements in controls and CS-exposed mice.
Notes: Compared with controls, FEV0.1s, FEV0.1s/FVC and Cdyn were significantly decreased in CS-exposed mice (A–C), but RL was elevated (D). Representative lung tissue section stained with hematoxylin–eosin of controls and CS-exposed mice (E), scale bars =100 μM; magnification = ×40. Compared with controls, MLI measurement was significantly increased in CS-exposed mice (F). Data in C and F panels are presented as mean ± SD; data of other panels are presented as median with range, n=8 for controls and n=8 for CS-exposed mice. **P<0.01 and ***P<0.001 represent significant differences compared to controls.
Abbreviations: CS, cigarette smoke; FEV0.1s, forced expiratory volume in 0.1s; FEV0.1s/FVC, ratio of FEV0.1s to forced vital capacity; Cdyn, dynamic lung compliance; RL, lung resistance; MLI, mean linear intercept.
![Figure 2 Lung function and histological measurements in controls and CS-exposed mice.Notes: Compared with controls, FEV0.1s, FEV0.1s/FVC and Cdyn were significantly decreased in CS-exposed mice (A–C), but RL was elevated (D). Representative lung tissue section stained with hematoxylin–eosin of controls and CS-exposed mice (E), scale bars =100 μM; magnification = ×40. Compared with controls, MLI measurement was significantly increased in CS-exposed mice (F). Data in C and F panels are presented as mean ± SD; data of other panels are presented as median with range, n=8 for controls and n=8 for CS-exposed mice. **P<0.01 and ***P<0.001 represent significant differences compared to controls.Abbreviations: CS, cigarette smoke; FEV0.1s, forced expiratory volume in 0.1s; FEV0.1s/FVC, ratio of FEV0.1s to forced vital capacity; Cdyn, dynamic lung compliance; RL, lung resistance; MLI, mean linear intercept.](/cms/asset/631cfa0f-054c-444e-b82b-c1799007b131/dcop_a_148595_f0002_c.jpg)
Figure 3 Downregulated MBD2 expression in CS-exposed mice.
Abbreviations: CS, cigarette smoke; MBD2, methyl-CpG-binding domain protein 2; IgG Ab, immunoglobulin G antibody.
![Figure 3 Downregulated MBD2 expression in CS-exposed mice.](/cms/asset/e060919c-3823-442b-9505-dd7815d9324f/dcop_a_148595_f0003_c.jpg)
Figure 4 CSE attenuated MBD2 expression in HBE cells in vitro.
Abbreviations: ANOVA, analysis of variance; CSE, cigarette smoke extract; HBE, human bronchial epithelial; MBD2, methyl-CpG-binding domain protein 2.
![Figure 4 CSE attenuated MBD2 expression in HBE cells in vitro.](/cms/asset/c5ae232c-963f-454d-8d92-aeb8dbf6969d/dcop_a_148595_f0004_b.jpg)
Figure 5 MBD2 knockdown increased IL-6 and IL-8 expression in HBE.
Abbreviations: Con-siRNA, negative control siRNA; HBE, human bronchial epithelial; IL, interleukin; MBD2, methyl-CpG-binding domain protein 2.
![Figure 5 MBD2 knockdown increased IL-6 and IL-8 expression in HBE.](/cms/asset/317074de-514b-45b4-9be8-4871c5e742fb/dcop_a_148595_f0005_b.jpg)
Figure 6 MBD2 knockdown enhanced ERK1/2 signal pathway-dependent IL-6 and IL-8 secretion by HBE.
Abbreviations: Con-siRNA, negative control siRNA; CSE, cigarette smoke extract; DMSO, dimethyl sulfoxide; HBE, human bronchial epithelial; IL, interleukin; MBD2, methyl-CpG-binding domain protein 2.
![Figure 6 MBD2 knockdown enhanced ERK1/2 signal pathway-dependent IL-6 and IL-8 secretion by HBE.](/cms/asset/05899ac8-87d5-4786-b836-282518275d56/dcop_a_148595_f0006_b.jpg)
Figure S1 Representative immunohistochemical image of cells in non-smokers’ lung tissues.
Notes: The red-boxed area indicates a region of higher magnification. The arrows indicate inflammatory cells and asterisks points to alveolar mesenchymal cells.
![Figure S1 Representative immunohistochemical image of cells in non-smokers’ lung tissues.Notes: The red-boxed area indicates a region of higher magnification. The arrows indicate inflammatory cells and asterisks points to alveolar mesenchymal cells.](/cms/asset/fbae735e-3267-4f64-8be1-b7289727aecc/dcop_a_148595_sf0001_c.jpg)
Figure S2 Levels of IL-6 and CXCL1 (KC) in BALF in controls and CS-exposed mice.
Notes: Compared with controls, levels of IL-6 (A) and CXCL1 (KC) (B) in BALF were increased in CS-exposed mice. Data are presented as means ± SD, n=8 for controls and n=8 for CS-exposed mice. P-values were calculated using Student’s t-test; **P<0.01 and ***P<0.001 represent significant differences compared to controls.
![Figure S2 Levels of IL-6 and CXCL1 (KC) in BALF in controls and CS-exposed mice.Notes: Compared with controls, levels of IL-6 (A) and CXCL1 (KC) (B) in BALF were increased in CS-exposed mice. Data are presented as means ± SD, n=8 for controls and n=8 for CS-exposed mice. P-values were calculated using Student’s t-test; **P<0.01 and ***P<0.001 represent significant differences compared to controls.](/cms/asset/3afef450-84fb-418b-b637-72acfcf51c35/dcop_a_148595_sf0002_b.jpg)