Physical exercise is effective in preventing cigarette smoke-induced pulmonary oxidative response in mice

Figures & data

Figure 1 Effects of physical training on morphological alterations in lung tissue after CS exposure.

Notes: Slices were made in thickness of 4 μm and stained with hematoxylin and eosin (A–C) or Weigert stain (D–F). (A, D) C (no pathologic alterations); (B, E) CS (emphysematous lesions); (C, F) CS+Ex (partial reduction of emphysema).
Abbreviations: C, control; CS, cigarette smoke; CS+Ex, cigarette smoke plus exercise.

Figure 2 (A) Hydroxyproline content and (B) superoxide production were evaluated in lung homogenates from mice exposed to CS and/or to Ex.

Notes: Results are presented as mean ± SD. Values of (A) are expressed in mg/g tissue and values of (B) are expressed in nmol/min/mg protein. The comparison between the groups was analyzed by ANOVA followed by the Tukey post hoc test. *In relation to C, P<0.05; **in relation to CS group, P<0.05.
Abbreviations: C, control; Ex, exercise; CS, cigarette smoke; SD, standard deviation; ANOVA, analysis of variance.

Figure 3 Superoxide dismutase (A), catalase (B), and glutathione peroxidase (C) activities in lung homogenates from mice exposed to CS and/or to Ex.

Notes: Results are presented as mean ± SD, and the results are expressed in U/mg protein. The comparison between the groups was analyzed by ANOVA followed by the Tukey post hoc test. *In relation to C, P<0.05; **in relation to CS group, P<0.05.
Abbreviations: C, control; Ex, exercise; CS, cigarette smoke; SD, standard deviation; ANOVA, analysis of variance.

Figure 4 Oxidative damage in lipids (A) and protein (B) in lung homogenates from mice exposed to CS and/or to Ex.

Notes: Results are presented as mean ± SD, and the results are expressed in nmol/mg protein. The comparison between the groups was analyzed by ANOVA followed by the Tukey post hoc test. *In relation to C, P<0.05; **in relation to CS group, P<0.05.
Abbreviations: TBars, 2-thiobarbituric acid reactive species; C, control; Ex, exercise; CS, cigarette smoke; SD, standard deviation; ANOVA, analysis of variance.