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Original Research

Spirolactone provides protection from renal fibrosis by inhibiting the endothelial–mesenchymal transition in isoprenaline-induced heart failure in rats

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Pages 1581-1588 | Published online: 05 May 2016

Figures & data

Table 1 Effect of spironolactone on cardiac functional index

Table 2 Effect of spironolactone on ventricular weight and kidney weight in an Iso-induced heart failure rat model

Figure 1 Hematoxylin and eosin staining of heart and kidney tissues in rats.

Notes: (A) Heart. (a) Control. (b) Iso. (c) Iso + low-dose spironolactone. (d) Iso + high-dose spironolactone. Magnification: ×200. (B) Kidney. (a) Control. (b) Iso. (c) Iso + low-dose spironolactone. (d) Iso + high-dose spironolactone. Magnification: ×400.
Abbreviation: Iso, isoprenaline.
Figure 1 Hematoxylin and eosin staining of heart and kidney tissues in rats.

Figure 2 Collagen areas of heart and kidney tissues in rats.

Notes: (A) Heart. (B) Kidney. (a) Control. (b) Iso. (c) Iso + low-dose spironolactone. (d) Iso + high-dose spironolactone. Magnification: ×200. *P<0.01 vs control; **P<0.01 vs Iso.
Abbreviation: Iso, isoprenaline.
Figure 2 Collagen areas of heart and kidney tissues in rats.

Table 3 Expression of Type I and III collagen in heart and kidney

Figure 3 Expression of CD31, α-SMA, and TGF-β1 proteins in rats.

Notes: (A) and (B) Immunofluorescence staining of effect of spironolactone in Iso-treated rats. (a) Control. (b) Iso. (c) Iso + low-dose spironolactone. (d) Iso + high-dose spironolactone. CD31 protein (endothelial phenotype) is stained red, α-SMA protein (myofibroblastic phenotype) is green, and nuclei are blue. Magnification: ×200. Western blot analysis of the protein levels (C) and (D) CD31 and α-SMA and (E) TGF-β1. *P<0.01 vs control, **P<0.01 vs Iso, ***P<0.05.
Abbreviations: CD31, cluster of differentiation-31; TGF-β1, transforming growth factor β1; α-SMA, α-smooth muscle actin; Iso, isoprenaline; GAPDH, glyceraldehyde- 3-phosphate dehydrogenase.
Figure 3 Expression of CD31, α-SMA, and TGF-β1 proteins in rats.