Figures & data
Table 1 Comparison values of IC50 of FLS, FLA, and FLB in MCF-7, MDA-MB-231 and MCF-10A cells
Table 2 The accession number and sequence of the primers used in the quantitative real-time PCR assay
Figure 1 The molecular structures of FLS, flavokawain A and flavokawain B.
![Figure 1 The molecular structures of FLS, flavokawain A and flavokawain B.](/cms/asset/973904af-038b-4918-864e-671d882332f4/dddt_a_12163088_f0001_b.jpg)
Figure 2 Light microscopic images of MCF-7.
Abbreviations: FLS: (E)-1-(2′-Hydroxy-4′,6′-dimethoxyphenyl)-3-(4-methylthio)phenyl)prop-2-ene-1-one; SE, standard error of the mean.
![Figure 2 Light microscopic images of MCF-7.](/cms/asset/e86c95cc-49e8-4546-948a-f144caca1f07/dddt_a_12163088_f0002_c.jpg)
Figure 3 Flow cytometry cell cycle and Annexin V analyses of control and FLS treated MCF-7 cells.
Abbreviations: FLS: (E)-1-(2′-Hydroxy-4′,6′-dimethoxyphenyl)-3-(4-methylthio)phenyl)prop-2-ene-1-one; SE, standard error of the mean; FITC+/PI+, late apoptosis population; FITC−/PI−, healthy cell population; FITC+/PI−, early apoptosis population; S, synthesis; RFU, relative fluorescence unit.
![Figure 3 Flow cytometry cell cycle and Annexin V analyses of control and FLS treated MCF-7 cells.](/cms/asset/33195a4c-e405-48bb-aa18-8e8c2c25a100/dddt_a_12163088_f0003_c.jpg)
Figure 4 qPCR analysis of apoptosis and cell cycle related genes; PLK1, WEE-1, c-Jun, GLUT in MCF-7 treated with FLS (36 μM) for 24, 48, and 72 hours.
Abbreviations: FLS: (E)-1-(2′-Hydroxy-4′,6′-dimethoxyphenyl)-3-(4-methylthio)phenyl)prop-2-ene-1-one; SE, standard error of the mean; qPCR, quantitative real time polymerase chain reaction.
![Figure 4 qPCR analysis of apoptosis and cell cycle related genes; PLK1, WEE-1, c-Jun, GLUT in MCF-7 treated with FLS (36 μM) for 24, 48, and 72 hours.](/cms/asset/dc91602e-f464-457c-af0a-9475d23e6153/dddt_a_12163088_f0004_b.jpg)
Figure 5 Differential protein expression of untreated and FLS treated MCF-7 cells.
Abbreviations: FLS: (E)-1-(2′-Hydroxy-4′,6′-dimethoxyphenyl)-3-(4-methylthio)phenyl)prop-2-ene-1-one; SE, standard error of the mean.
![Figure 5 Differential protein expression of untreated and FLS treated MCF-7 cells.](/cms/asset/16732290-be13-4464-93b8-d5d3b171d36c/dddt_a_12163088_f0005_b.jpg)
Figure 6 Detection of the activation of caspase 9 and cytochrome c in MCF-7 treated with FLS (36 μM) for 24, 48, and 72 hours.
Abbreviations: FLS: (E)-1-(2′-Hydroxy-4′,6′-dimethoxyphenyl)-3-(4-methylthio) phenyl)prop-2-ene-1-one; SE, standard error of the mean.
![Figure 6 Detection of the activation of caspase 9 and cytochrome c in MCF-7 treated with FLS (36 μM) for 24, 48, and 72 hours.](/cms/asset/3a98a52a-e2b8-4242-8c6f-6dd5d394728c/dddt_a_12163088_f0006_b.jpg)