Role of glycogen synthase kinase-3β inhibitor AZD1080 in ovarian cancer

Figures & data

Figure 1 Effects of AZD1080 on ovarian carcinoma cell proliferation and cell cycle.

Notes: (A) MTT cell proliferation assays show that AZD1080 suppressed A2780 and OVCAR3 cell proliferation in a concentration-dependent manner. (B) AZD1080 induced G1 arrest in the two cell lines and was dose dependent. *P<0.05. Results represent three independent experiments; data are expressed as mean ± SD. S, G1, G2 represent synthesis phase, pre-synthetic gap 1 period, and post-synthetic gap 2 period, respectively.
Abbreviations: MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DMSO, dimethyl sulfoxide; h, hour; SD, standard deviation.

Figure 2 Effects of AZD1080 on ovarian carcinoma metastatic ability.

Notes: Wound healing assays show that AZD1080 decreased the migration ability of A2780 and OVCAR3 cells in a dose-dependent manner. *P<0.05. Results represent three independent experiments; data are expressed as mean ± SD.
Abbreviations: DMSO, dimethyl sulfoxide; h, hour; SD, standard deviation.

Figure 3 Effects of AZD1080 on ovarian carcinoma cell invasion.

Notes: Transwell assays show that AZD1080 decreased the invasion ability of A2780 and OVCAR3 cells in a dose-dependent manner. *P<0.05. Results represent three independent experiments; data are expressed as mean ± SD.
Abbreviations: DMSO, dimethyl sulfoxide; h, hour; SD, standard deviation.

Figure 4 Effects of AZD1080 on ovarian carcinoma cell lamellipodia formation.

Note: AZD1080 inhibited A2780 and OVCAR3 cell lamellipodia formation (×40), as indicated by the loss of F-actin structure, in a dose-dependent manner. *P<0.05. Results represent three independent experiments; data are expressed as mean ± SD.
Abbreviation: DMSO, dimethyl sulfoxide.

Figure 5 mRNA and protein expression in AZD1080-treated ovarian carcinoma cells.

Notes: AZD1080 exposure reduced the mRNA and protein expression of GSK-3β, CDK2, CDK1, cyclin D1, MMP9, and Bcl-xL in ovarian carcinoma cell lines as determined with real-time RT-PCR (A) and Western blot (B), respectively. *P<0.05. Results represent three independent experiments; data are expressed as mean ± SD.
Abbreviations: mRNA, messenger RNA; GSK-3β, glycogen synthase kinase-3β; CDK, cyclin-dependent kinase; MMP9, matrix metalloproteinase-9; Bcl-xL, B-cell lymphomaextra large; RT-PCR, reverse transcription polymerase chain reaction; DMSO, dimethyl sulfoxide; SD, standard deviation.