MART-10, a newly synthesized vitamin D analog, represses metastatic potential of head and neck squamous carcinoma cells

Figures & data

Figure 1 1α,25(OH)₂D₃ and MART-10 inhibited FaDu cell migration and invasion.

Notes: The migration (A) and invasion (B) of FaDu cells, pretreated with 1α,25(OH)₂D₃ or MART-10 for 48 hours, were measured by trans-well migration assay and matrigel invasion assay, respectively. Twenty-four hours or 48 hours were allowed for cells to migrate or invade. Cells that passed through filters were counted under the microscope (IX71). Experiments were performed in triplicate and repeated at least three times (*P<0.05 and **P<0.01).
Abbreviation: MART-10, 19-Nor-2α-(3-hydroxypropyl)-1α,25(OH)₂D₃.

Figure 2 The effects of 1α,25(OH)₂D₃ and MART-10 on the migration and invasion of SCC-25 cells.

Notes: The migration (A) and invasion (B) of SCC-25 cells, which were pretreated with 1α,25(OH)₂D₃ or MART-10 for 48 hours, were measured by trans-well migration assay and matrigel invasion assay, respectively. Twenty-four hours or 48 hours were allowed for cells to migrate or invade. Cells that passed through filters were counted under the microscope (IX71). Experiments were performed in triplicate and repeated at least three times (*P<0.05 and **P<0.01).
Abbreviation: MART-10, 19-Nor-2α-(3-hydroxypropyl)-1α,25(OH)₂D₃.

Figure 3 Effects of 1α,25(OH)₂D₃ or MART-10 on the expressions of Snail, Slug, and Twist, in FaDu cells.

Notes: (A) Western blot showing the expressions of Snail, Slug, and Twist in FaDu cells after 2 days of 10⁻⁷ M 1α,25(OH)₂D₃ or MART-10 treatment. Tubulin was used as the loading control. (B) Quantitative result of Snail, Slug, and Twist expressions in FaDu cells with or without treatment. Each value is a mean ± SD of three independent determinations (*P<0.05 and **P<0.01).
Abbreviation: MART-10, 19-Nor-2α-(3-hydroxypropyl)-1α,25(OH)₂D₃.

Figure 4 1α,25(OH)₂D₃ or MART-10 induced an increase in E-cadherin but a decrease in N-cadherin expression in FaDu cells.

Notes: (A) Western blot depicting the expressions of E-cadherin and N-cadherin in FaDu cells after 10⁻⁷ M 1α,25(OH)₂D₃ or MART-10 treatment for 2 days. Tubulin was used as the loading control. (B) Quantitative result of the Western blot showing E-cadherin and N-cadherin expressions in FaDu cells after 2 days of 10⁻⁷ M 1α,25(OH)₂D₃ or MART-10 treatment. Each value is a mean ± SD of three independent determinations (*P<0.05).
Abbreviation: MART-10, 19-Nor-2α-(3-hydroxypropyl)-1α,25(OH)₂D₃.

Figure 5 Effects of 1α,25(OH)₂D₃ and MART-10 on intracellular expression and extracellular activity of MMP-2 and MMP-9 in FaDu cells.

Notes: (A) Western blot depicting the expressions of MMP-2 and MMP-9 in FaDu cells after 2 days of 10⁻⁷ M 1α,25(OH)₂D₃ or MART-10 treatment. Tubulin was used as the loading control. (B) Quantitative analysis of MMP-2 and MMP-9 expressions in FaDu cells after treatment. Each value was a mean ± SD of three independent determinations (*P<0.05). (C) The extracellular MMP-2 and MMP-9 activities of FaDu cells were analyzed by zymography. After 2 days of treatment, conditioned media were collected for activity assay. The digested areas (white bands) corresponded to the activities of MMP-2 and MMP-9.
Abbreviation: MART-10, 19-Nor-2α-(3-hydroxypropyl)-1α,25(OH)₂D₃.