Dietary arginine silicate inositol complex increased bone healing: histologic and histomorphometric study

Figures & data

Table 1 The effects of ASI on histomorphometric changes

Histomorphometic parameters	Time (days)a	Control (n=21)	ASI (n=21)	P-value
NBF	7	0	0	NA
NBF	14	0	0	NA
NBF	28	1.14±0.38	1.57±0.53*	<0.001
OB	7	35.86±2.55	40.14±3.02	<0.106
OB	14	33.86±2.68	38.14±2.41*	<0.001
OB	28	30.29±1.8	34.58±2.64*	<0.001
OC	7	3.86±0.69	7.29±0.76*	<0.001
OC	14	2.14±0.69	4.14±1.21*	<0.001
OC	28	0.29±0.49	0.57±0.53*	<0.001

Notes: Data are presented as mean ± standard deviation.

* Significant difference between the without ASI group (control) and the with ASI group (ASI) (t-test).

a Days after the calvarial critical-sized defect was created.

Abbreviations: ASI, arginine silicate inositol complex; NA, not applicable; NBF, new bone formation; OB, osteoblast; OC, osteoclast.

Figure 1 Histopathologic sections of both the groups at 7 days.

Notes: Control: (A) 20× magnification, (B) 40× magnification, (C) 100× magnification; ASI: (D) 20× magnification, (E) 40× magnification, (F) 100× magnification. Osteoclast morphology was scored as follows: 0, no osteoclasts; 1, mild osteoclasts; and 2, dense osteoclasts. Bone formation was scored as follows: 0, no bone formation; 1, mild visible bone formation; 2, moderate visible bone formation; and 3, dense visible bone formation.
Abbreviation: ASI, arginine silicate inositol complex.

Figure 2 Histopathologic sections of both the groups at 14 days.

Notes: Control: (A) 20× magnification, (B) 40× magnification, (C) 100× magnification; ASI: (D) 20× magnification, (E) 40× magnification, (F) 100× magnification. Osteoclast morphology was scored as follows: 0, no osteoclasts; 1, mild osteoclasts; and 2, dense osteoclasts. Bone formation was scored as follows: 0, no bone formation; 1, mild visible bone formation; 2, moderate visible bone formation; and 3, dense visible bone formation.
Abbreviation: ASI, arginine silicate inositol complex.

Figure 3 Histopathologic sections of both the groups at 28 days.

Notes: Control: (A) 20× magnification, (B) 40× magnification, (C) 100× magnification; ASI: (D) 20× magnification, (E) 40× magnification, (F) 100× magnification. Osteoclast morphology was scored as follows: 0, no osteoclasts; 1, mild osteoclasts; and 2, dense osteoclasts. Bone formation was scored as follows: 0, no bone formation; 1, mild visible bone formation; 2, moderate visible bone formation; and 3, dense visible bone formation.
Abbreviation: ASI, arginine silicate inositol complex.