Radiolabeled tirofiban – a potential radiopharmaceutical for detection of deep venous thrombosis

Figures & data

Figure 1 Diagram of freeze drying process.

Figure 2 Effect of in vitro Tf on ADP-induced aggregation of human platelets.

Notes: (A) Effect of ADP (1 μM, 10 μM, or 100 μM). (B) Inhibition of the effect of 10 μM of ADP by increasing the concentrations of Tf (10 nM to 2 μM). (C) Inhibition by 10 nM of Tf of the ADP effect in respect to the timing of Tf addition. Suspended human platelets (450–475 μL) were incubated with ADP with or without Tf for 5 minutes.
Abbreviations: ADP, adenosine diphosphate; Tf, tirofiban.

Figure 3 Mean serum concentrations of unlabeled (A) and of ^99mTc-tirofiban (B) over 60 minutes after intravenous injection in rats without or with induced DVT.

Notes: (A) Rats (n=6/group) were injected (tail vein) with unlabeled tirofiban (0.6 mg/kg), and serum concentrations (mg/L) were determined by a validated high performance liquid chromatography. (B) Rats (n=6/group) were injected (tail vein) with ^99mTc-tirofiban (3–4×10⁵ cpm, corresponding to 2 nmol of tirofiban). The mean elimination half-lives (t_1/2) are depicted.
Abbreviation: DVT, deep venous thrombosis.

Figure 4 Whole body scintigraphy at 30 minutes (A) and at 60 minutes (B) after injection (tail vein) of ^99mTc-tirofiban (3–4×105 cpm, corresponding to 2 nmol of tirofiban) to rats with induced DVT.

Notes: Arrows depict the sites of accumulation of radioactivity. A typical scintigram is shown. Copyright ©2015 AE Press s.r.o. Reproduced from Darkovska SM, Janevik-Ivanovska E, Ugresic N, Djorgoski I. Imaging of deep venous thrombosis using radioactive-labeled tirofiban. Bratisl Med J. 2015;116(10):621–626.³⁷
Abbreviation: DVT, deep venous thrombosis.

DarkovskaSMJanevik-IvanovskaEUgresicNDjorgoskiIImaging of deep venous thrombosis using radioactive-labeled tirofibanBratisl Med J2015116106216266

 Google Scholar