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Original Research

Effect of picroside II on hind limb ischemia reperfusion injury in rats

, , , , , , , , & show all
Pages 1917-1925 | Published online: 26 Jun 2017

Figures & data

Table 1 Oxidant status parameters in rat skeletal muscle tissue (mean ± standard deviation)

Table 2 TUNEL positive cell data in skeletal muscle of rats (mean ± standard deviation)

Figure 1 In the micrograph of the control group, brown staining is observed in a small number of cells showing immunoreaction by TUNEL staining. Magnifi cation: ×400.

Figure 1 In the micrograph of the control group, brown staining is observed in a small number of cells showing immunoreaction by TUNEL staining. Magnifi cation: ×400.

Figure 2 In the micrograph of the picroside group, brown staining is observed in a small number of cells showing immunoreaction by TUNEL staining. Magnification: ×400.

Figure 2 In the micrograph of the picroside group, brown staining is observed in a small number of cells showing immunoreaction by TUNEL staining. Magnification: ×400.

Figure 3 In the micrograph of the ischemia reperfusion group, brown staining is observed in apoptotic cell nuclei showing immunoreaction by TUNEL staining. Magnification: ×400.

Figure 3 In the micrograph of the ischemia reperfusion group, brown staining is observed in apoptotic cell nuclei showing immunoreaction by TUNEL staining. Magnification: ×400.

Figure 4 In the micrograph of the ischemia reperfusion + picroside II group, brown staining is observed in apoptotic cell nuclei showing immunoreaction by TUNEL staining. Magnification: ×400.

Figure 4 In the micrograph of the ischemia reperfusion + picroside II group, brown staining is observed in apoptotic cell nuclei showing immunoreaction by TUNEL staining. Magnification: ×400.

Figure 5 The cross-sectional faces of striated skeletal muscle fibers are observed in a multi-sided appearance in the micrograph of the control group. Peripheral nuclei (arrow) under the sarcolemma of muscle fibers, cross sections of myofibrils (asterisk) in the cytoplasm are observed and in the perimysium (P) surrounding the fascicles formed by muscle fibers, blood vessels (arrowheads) draw attention. H&E ×200.

Figure 5 The cross-sectional faces of striated skeletal muscle fibers are observed in a multi-sided appearance in the micrograph of the control group. Peripheral nuclei (arrow) under the sarcolemma of muscle fibers, cross sections of myofibrils (asterisk) in the cytoplasm are observed and in the perimysium (P) surrounding the fascicles formed by muscle fibers, blood vessels (arrowheads) draw attention. H&E ×200.

Figure 6 The cross-sectional faces of striated skeletal muscle fibers are observed in a multi-sided appearance in the micrograph of the picroside group. Peripheral nuclei (arrow) under the sarcolemma of muscle fibers and blood vessels (arrowheads) in the perimysium (P) surrounding the fascicles formed by muscle fibers are observed. H&E ×200.

Figure 6 The cross-sectional faces of striated skeletal muscle fibers are observed in a multi-sided appearance in the micrograph of the picroside group. Peripheral nuclei (arrow) under the sarcolemma of muscle fibers and blood vessels (arrowheads) in the perimysium (P) surrounding the fascicles formed by muscle fibers are observed. H&E ×200.

Figure 7 In the micrograph of the ischemia reperfusion group, the cross-sectional faces of the muscle fibers are observed to have a rounded (arrow) shape. Increase in endomysium distance between the fibers (star) and atrophy in the muscle fibers (tailed arrow) are also observed. H&E ×200.

Figure 7 In the micrograph of the ischemia reperfusion group, the cross-sectional faces of the muscle fibers are observed to have a rounded (arrow) shape. Increase in endomysium distance between the fibers (star) and atrophy in the muscle fibers (tailed arrow) are also observed. H&E ×200.

Figure 8 Cross-sections of the fibers in the micrograph of the ischemia reperfusion group show a rounded shape of the cross-sections and middle position of the core (arrow), advanced atrophy in the muscle fibers are observed. H&E ×400.

Figure 8 Cross-sections of the fibers in the micrograph of the ischemia reperfusion group show a rounded shape of the cross-sections and middle position of the core (arrow), advanced atrophy in the muscle fibers are observed. H&E ×400.

Figure 9 In cross-sections of muscle fibers of the micrograph of the ischemia reperfusion group, some cross-sectional faces take round shape (arrow) and mononuclear cell infiltration (thick arrow), vessel (arrowhead) and peripheral nerve sections (tailed arrow) are observed in the thickening perimysium (P) H&E ×200.

Figure 9 In cross-sections of muscle fibers of the micrograph of the ischemia reperfusion group, some cross-sectional faces take round shape (arrow) and mononuclear cell infiltration (thick arrow), vessel (arrowhead) and peripheral nerve sections (tailed arrow) are observed in the thickening perimysium (P) H&E ×200.

Figure 10 Cross-sections of the fibers in the micrograph of the ischemia reperfusion + picroside II group, show a rounded shape of the cross-sections (arrowhead) and middle position of the core (arrow), mononuclear cell infiltration (tailed arrow) between muscle fibers are observed. In some areas, muscle fiber sections (asterisk) showing a normal, multifaceted cross-sectional surface are observed. H&E ×200.

Figure 10 Cross-sections of the fibers in the micrograph of the ischemia reperfusion + picroside II group, show a rounded shape of the cross-sections (arrowhead) and middle position of the core (arrow), mononuclear cell infiltration (tailed arrow) between muscle fibers are observed. In some areas, muscle fiber sections (asterisk) showing a normal, multifaceted cross-sectional surface are observed. H&E ×200.

Figure 11 Cross-sections of the fibers in the micrograph of the ischemia reperfusion + picroside II group, show a rounded shape of the cross-sections (arrowhead) and increase in distance between muscle fibers (star), atrophic muscle fibers (arrow) are observed. In some areas, muscle fiber sections (asterisk) showing a normal, multifaceted cross-sectional surface are observed. H&E ×200.

Figure 11 Cross-sections of the fibers in the micrograph of the ischemia reperfusion + picroside II group, show a rounded shape of the cross-sections (arrowhead) and increase in distance between muscle fibers (star), atrophic muscle fibers (arrow) are observed. In some areas, muscle fiber sections (asterisk) showing a normal, multifaceted cross-sectional surface are observed. H&E ×200.