Protective effects of honokiol on ischemia/reperfusion injury of rat ovary: an experimental study

Figures & data

Figure 1 Bilateral ovaries after midline laparotomy in the sham group.

Figure 2 I/R injury performed in ovaries.

Abbreviation: I/R, ischemia/reperfusion.

Figure 3 The appearance of the ovaries after honokiol.

Table 1 Histopathologic evaluation scores of rat ovarian tissues in all groups (n=8)

Histopathologic scores	Vascular congestion (mean ± SD)	Hemorrhage (mean ± SD)	Inflammatory cell infiltration (mean ± SD)	Cellular degeneration (mean ± SD)	Total score (mean ± SD)
Sham group (I)	0.4±0.5	0.1±0.3	0.1±0.3	0.1±0.3	0.8±0.7
Torsion group (II)	1.5±0.5	1.5±0.9	1.0±0.0	0.6±0.5	4.6±1.1
Detorsion group (III)	2.2±0.5	2.4±0.5	1.5±0.5	1.6±0.5	7.7±1.3
Saline group (IV)	2.4±0.5	2.8±0.5	1.6±0.5	1.6±0.5	8.4±1.9
Honokiol group (V)	1.6±0.7	1.0±0.8	0.5±0.5	0.5±0.5	3.6±1.3
P-values
II→I	0.003	0.001	0.000	0.046	0.001
III→I	0.000	0.000	0.000	0.001	0.001
IV→I	0.000	0.000	0.000	0.001	0.001
V→I	0.004	0.003	0.025	0.117	0.002
III→II	0.015	0.041	0.025	0.005	0.002
IV→II	0.010	0.007	0.009	0.005	0.001
V→II	0.814	0.264	0.025	0.626	0.157
IV→III	0.602	0.143	0.626	0.100	0.230
V→III	0.061	0.003	0.006	0.003	0.001
V→IV	0.040	0.001	0.003	0.003	0.001

Note: Data in bold indicates statistical significance at P<0.005.

Abbreviation: SD, standard deviation.

Figure 4 H&E-stained ovarian sections (×200).

Notes: (A) Sham: rat ovarian tissue that is close to normal in the sham group. (B) Torsion: severe congestion in rat ovary in the torsion group. (C) Detorsion: severe congestion and hemorrhage in rat ovary in the detorsion group. (D) Saline: severe congestion and hemorrhage in rat ovary in the saline group. (E) Honokiol: mild congestion in rat ovary in the honokiol group.
Abbreviation: H&E, hematoxylin and eosin.

Table 2 MDA and NO concentrations in all groups (n=8)

	MDA (ovarian tissue) (nmol/mg) (mean ± SD)	NO (ovarian tissue) (nmol/mg) (mean ± SD)	MDA (plasma) (nmol/mL) (mean ± SD)	NO (plasma) (nmol/mL) (mean ± SD)
Sham (I)	11.1±0.4	14.2±0.5	14.1±3.3	132.9±72.8
Torsion (II)	12.06±0.4	15.3±0.4	14.4±4.9	136.4±109.1
Detorsion (III)	12.02±0.3	14.8±0.6	12.2±1.7	68.5±18.4
Saline (IV)	11.02±2.3	13.5±2.0	14.6±4.1	111.4±84.9
Honokiol (V)	10.5±1.0	11.9±1.8	14.1±1.9	278.1±122.6
P-values
II→I	0.003	0.002	0.916	0.834
III→I	0.001	0.040	0.318	0.046
IV→I	0.360	0.958	0.916	0.401
V→I	0.227	0.036	0.006	0.012
II→III	0.833	0.207	0.401	0.529
II→IV	0.141	0.005	0.834	0.753
II→V	0.002	0.003	0.046	0.046
III→IV	0.172	0.093	0.294	0.713
III→V	0.001	0.009	0.009	0.001
IV→V	0.036	0.141	0.012	0.012

Note: Data in bold indicates statistical significance at P<0.005.

Abbreviations: MDA, malondialdehyde; NO, nitric oxide; SD, standard deviation.