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Original Research

The Effect of Rooibos (Aspalathus linearis), Honeybush (Cyclopia intermedia) and Sutherlandia (Lessertia frutescens) on Testicular Insulin Signalling in Streptozotocin-Induced Diabetes in Wistar Rats

ORCID Icon, ORCID Icon & ORCID Icon
Pages 1267-1280 | Published online: 19 Mar 2021

Figures & data

Table 1 Biometric Data

Table 2 Body, Organ, and Tissue Weights

Table 3 Total Polyphenolic Contents

Figure 1 Intraperitoneal glucose tolerance test. (A) Glucose tolerance test, (B) correlation between fasting blood glucose levels and fluid intake per day.

Abbreviations: DC, diabetic control; DRF, diabetic +rooibos; DHB, diabetic + honeybush; DSL, diabetic +Sutherlandia.
Figure 1 Intraperitoneal glucose tolerance test. (A) Glucose tolerance test, (B) correlation between fasting blood glucose levels and fluid intake per day.

Figure 2 Protein expression of phospho and total ERK1/2. (A) individual phospho: total ERK1/2, (B) p/tERK1/2 at a glance, (C) probed blot. *p<0.05 vs control. n, 4–5.

Abbreviations: Veh, vehicle; DC, diabetic control; DRF, diabetic + rooibos; DHB, diabetic + honeybush; DSL, diabetic + Sutherlandia.
Figure 2 Protein expression of phospho and total ERK1/2. (A) individual phospho: total ERK1/2, (B) p/tERK1/2 at a glance, (C) probed blot. *p<0.05 vs control. n, 4–5.

Figure 3 Protein expression of total IRS-1 and IRS-2. (A) Ratio of IRS1 to IRS 2, (B) probed blot. n=4–5.

Abbreviations: Veh, vehicle; DC, diabetic control; DRF, diabetic + rooibos; DHB, diabetic + honeybush; DSL, diabetic +Sutherlandia.
Figure 3 Protein expression of total IRS-1 and IRS-2. (A) Ratio of IRS1 to IRS 2, (B) probed blot. n=4–5.

Figure 4 Total protein expression of PkB/Akt. (A) Graph of PkB/Akt, (B) probed blot of PkB/Akt. Values in bracket () denotes percentage change versus DC. n=4–5.

Abbreviations: Veh, vehicle; DC, diabetic control; DRF, diabetic + rooibos; DHB, diabetic + honeybush; DSL, diabetic + Sutherlandia.
Figure 4 Total protein expression of PkB/Akt. (A) Graph of PkB/Akt, (B) probed blot of PkB/Akt. Values in bracket () denotes percentage change versus DC. n=4–5.

Figure 5 Total protein expression of GLUT4. (A) Graph showing tGLUT4, (B) probed blot. Values in bracket () denotes percentage change versus DC. n=4–5.

Abbreviations: Veh, vehicle; DC, diabetic control; DRF, diabetic + rooibos; DHB, diabetic + honeybush; DSL, diabetic +Sutherlandia.
Figure 5 Total protein expression of GLUT4. (A) Graph showing tGLUT4, (B) probed blot. Values in bracket () denotes percentage change versus DC. n=4–5.

Figure 6 Plasma levels of testosterone and estradiol. (A) Testosterone, (B) estradiol. Values in bracket () denotes percentage change versus DC. n=4–5.

Abbreviations: Veh, vehicle; DC, diabetic control; DRF, diabetic + rooibos; DHB, diabetic + honeybush; DSL, diabetic + Sutherlandia.
Figure 6 Plasma levels of testosterone and estradiol. (A) Testosterone, (B) estradiol. Values in bracket () denotes percentage change versus DC. n=4–5.