Current Aspects in the Biology, Pathogeny, and Treatment of Candida krusei, a Neglected Fungal Pathogen

Figures & data

Figure 1 Candida krusei cell and colony morphology. (A) Yeast cells were grown in YPD broth until reach the exponential phase and then stained with calcofluor white, to label chitin. Scale bar = 10 µm. The arrowheads indicate the mother cells. (B) Cell filamentation was stimulated in RPMI medium incubated at 37°C. Scale bar = 20 µm. (C) A C. krusei colony grown on a YPD plate. Scale bar = 5.0 mm. Images from panels A and B were taken with a Zeiss Axioscope-40 microscope and an Axiocam MRc camera.

Table 1 Prediction of Some Virulence Factors in Candida krusei

Virulence Factor	C. albicans Gene	C. krusei Gene*	E value**	Similarity (%)**
Adhesins	EAP1	No found	–	–
	IFF4	No found	–	–
	MP65	ONH75632	5e^−118	66
		ONH70941	1e^−68	55
		ONH73292	8e^−42	55
	PHR1	ONH72606	0.0	72
	INT1	ONH72359	2e^−57	53
	ALS	No found	–	–
	ECM33	No found	–	–
Secreted hydrolases	PLB1-PLB5	ONH77577	1e^−166	61
		ONH74522	2e^−88	51
	LIP5 or LIP8	No found	–	–
	SAP1 – SAP5	ONH77652	2e^−39	47
		ONH72963	4e^−22	45
		ONH70287	2e^−22	42
		ONH77630	5e^−20	46
		ONH77640	7e^−10	55
Biofilms	HSP90	ONH74083	4e^−123	92
	BCR1	ONH74628	8e^−29	70
	EFG1	ONH73730	9e^−66	92
	ROB1	No found	–	–
	BRG1	OUT23966	9e^−21	67
	ZAP1	OUT21350	2e^−59	52
Dimorphism	HGC1	AWU73609	4e^−16	45
	NRG1	ONH70717	1e^−27	64
	TUP1	ONH77322	0.0	67
	CPH1	OUT20780	2e^−79	59

Notes: *Gene nomenclature corresponds to accession codes of the GeneBank database (https://www.ncbi.nlm.nih.gov/genbank/). **When comparing the encoded protein of C. krusei gene with the putative ortholog in Candida albicans.