Genetic Characterization of Colistin-Resistant Salmonella enterica ST34 Co-Harbouring Plasmid-Borne mcr-1, bla_CTX-M-15 and bla_KPC-2 Recovered from a Paediatric Patient in Shenzhen, China

Figures & data

Table 1 Antimicrobial Susceptibility of Salmonella Sp-15-127, Transconjugant’s and E. Coli C₆₀₀ (Recipient)

Isolates	MIC (mg/mL)
	AMK	ATM	FT	CT	CIP	CRO	TZP	GEN	FEP	CRO	CAZ	TM	IPM	LEV	SXT
Salmonella sp-15-127 ATCC25922 Transconjugant’s E. coli C600 (recipient)	16	≤1	128	8	≥4	≥64	≥128	≥32	≤1	≥64	≥64	≥16	16	≥16	≤20
	≤2 ≤2	≤2 ≤2	≤1 16	≤0.25 4	≤1 2	≤1 ≥64	≤1 64	≤1 ≤16	≤1 ≤1	≤1 ≥64	≤1 ≥64	≤1 ≥16	≤1 8	≤1 ≤1	≤1 ≤20
	≤2	≤2	≤1	≤0.25	≤1	≤1	≤1	≤1	≤1	≤1	≤1	≤1	≤1	≤1	≤20

Abbreviations: AMK, amikacin; ATM, aztreonam; FT, nitrofurantoin; CT, colistin; CIP, ciprofloxacin; CRO, ceftriaxone; TZP, piperacillin; GEN, gentamycin; FEP, cefepime; CRO, ceftriaxone; CAZ, ceftazidime; TM, tobramycin; IPM, imipenem; LEV, levofloxacin; SXT, trimethoprim/sulfamethoxazole.

Figure 1 S1-PFGE pattern for SP-15-127 strain and Southern blot analysis of mcr-1 genes.

Notes: Lane-L: Lamba marker, Lane 1: PFGE result of I-CeuI-digested DNA of SP-15-127; Lane 2: PFGE patterns for I-CeuI-digested DNA of the transconjugants C₆₀₀; Lane 3 and 4: Southern hybridization with the probes specific to the mcr-1 gene.