Comparative Proteomic Analysis Reveals Antibacterial Mechanism of Patrinia scabiosaefolia Against Methicillin Resistant Staphylococcus epidermidis

Figures & data

Table 1 Proteins Chosen for Investigation of DEPs in MRSE Under PS Stress

Protein ID	Portein Name	Gene Name	Average iTRAQ Ratio	Main Function
U6C447	Arginine deiminase	arcA	0.633	Arginine deiminase cataly arginine to citrulline.
A0A7I0AL81	Phenylalanine–tRNA ligase beta subunit	PheT	0.752	Phenylalanine–tRNA ligase beta subunit plays crucial roles in protein biosynthesis by catalyzing the synthesis of aminoacyl-tRNAs.
D4FNT3	Protein DltD	dltD	0.58	Protein DltD is necessary for incorporation of d-Ala into lipoteichoic acid, which is one of anionic polymers to compose cell wall.
A0A3G1RLZ5	Accumulation-associated protein	SasG	0.682	Accumulation-associated protein, as a family of surface proteins, were responsible for the initial attachment with host cells and that attachment regarding as important step in biofilm formation.

Table 2 Primers for RT-PCR

Gene Name	Primer Sequences	Size (bp)	Annealing Temp (°C)
arcA	F:5-TGTAAAATAAAGGTTGGGC-3	121bp	60
	R:5-AAATAAAATCATGGCTGGC-3
PheT	F:5-GAGAAGGACCAATAGT-3	112bp	60
	R:5-AATGAAAGTCAAGAAT-3
dltD	F:5-CATTGGGATGTTTTGC-3	296bp	60
	R:5-GTTCCTTATTGGTGCG-3
SasG	F:5-TAAGGACATAAACGGA-3	108bp	60
	R:5-TCTAAAAACGAGGAAT-3

Figure 1 Significantly differential proteins of MRSE in 2.5mg/mL PS stress using TMT-based quantitative proteomics. (A) The horizontal axis is the relative quantitative protein value after Log2 logarithm conversion, and the vertical axis is the difference significance test p-value value after -Log10 logarithm conversion. The red dots indicate up-regulated proteins, and blue dots indicate down-regulated proteins. (B) The number of DEPs. Q1 (<0.769) and Q2 (0.769–0.833) represent down-regulated proteins, and Q3 (1.2–1.3) and Q4 (>1.3) represent up-regulated proteins.

Figure 2 Continued.

Figure 2 Continued.

Figure 2 Go annotation and KEGG pathway of DEPs: gene ontology terms for subcellular location distribution. (A) cellular component (B) biological process (C) molecular function (D) main KEGG pathway.

Figure 3 String network of DEPs of MRSE in PS stress. Colored lines between the proteins indicate the various types of interaction evidence. Structure which is drawn in the protein nodes indicated the availability of 3D protein structure information. (A) String network of up-regulated proteins (B) String network of down-regulated proteins.

Figure 4 The mRNA levels of four genes were respectively analyzed by qPCR method in MRSE with and without PS.