Antimicrobial Profiles and Conventional PCR Assay of Shiga Toxigenic Escherichia coli O157:H7 (STEC) Isolated from Cattle Slaughtered at Bedele Municipal Abattoir, South West Ethiopia

Figures & data

Table 1 Prevalence of Escherichia coli O157:H7 from Different Types of Samples

Types of Samples	Number of Samples	E.coli (%)	E.coli O157:H7 (%)
Feacal sample	150	23(15.3%)	7(4.7%)
Carcass swab	150	17(11.3%)	5(3.3%)
Hand swabs	93	7(7.5%)	1(1.1%)
Knife swabs	93	4(4.3%)	1(1.1%)
Water samples	30	1(3.3%)	–
Total(Overall)	516	52(10.1%)	14(2.7%)

Table 2 Statistical Association of Prevalence of E. coli O157:H7 with Different Risk Factors

Risk Factors	Number	E. coli	E. coli O157:H7 (%)	Chi-Square (χ2)	P-value
Sex
Male	137	36(26.3%)	11(8%)	10.32	0.07
Female	13	4(30.8%)	1(7.7%)
Age
Young (2≤3 years)	6	3(50%)	1(16.7%)	7.16	0.002
Adult (3–6 years)	121	28(23.1%)	9(7.4%)
Old (≥6 years)	23	9(39.1%)	2(8.7%)
Body condition
Thin	9	2(22.2%)	2(22.2%)	18.23	0.103
Medium	111	31(27.9%)	7(6.3%)
Good	30	7 (23.3%)	3(10%)

Table 3 Antimicrobial Sensitivity Patterns of Serologically Positive Isolates of E. coli O157:H7

Anti. Used	Types of Samples									Aver. Antimicrobial Sensitivity Patterns from All Sample Types
	Feacal Samples(n=7)			Carcass Samples(n=5)			Hand and Knife Swab Sample(n=2)
	S	I	R	S	I	R	S	I	R	S	I	R
	No. %	No.%	No.%	No.%	No.%	No.%	No.%	No.%	No.%
AMP	1(14.3%)	2(28.6%)	4(57.1%)	0(%)	2(40%)	3(60%)	0(%)	0(%)	1(50%)	4.80%	22.9%	55.7%
CEP	5(71.4%)	1(14.3%)	1(14.3%)	3(60%)	1(20%)	0(%)	1(50%)	0(%)	0(%)	60.5%	11.4%	4.80%
CIP	7(100%)	0(0%)	0(0%)	4(80%)	1(20%)	0(%)	0(%)	0(%)	0(%)	60.0%	6.70%	0.00%
C	5(71.4%)	2(28.6%)	0(0%)	3(60%)	1(20%)	1(20%)	0(%)	0(%)	0(0%)	43.8%	16.2%	6.70%
GEN	4(57.1%)	3(42.9%)	0(0%)	2(40%)	3(60%)	0(%)	1(50%)	0(%)	0(0%)	52.0%	34.3%	0.00%
KAN	6(85.7%)	1(14.3%)	0(%)	5(100%)	0(%)	0(%)	0(%)	1(50%)	0(0%)	61.9%	21.4%	0.00%
NA	3(42.9%)	3(42.9%)	1(14.3%)	2(40%)	3(60%)	0(%)	0(%)	0(%)	2(100%)	27.6%	34.3%	38.1%
SXT	5(71.4%)	1(14.3%)	1(14.3%)	4(80%)	1(20%)	0(%)	2(100%)	0(%)	0(0%)	83.8%	11.4%	4.8%
S	2(28.6%)	1(14.3%)	4(57.1%)	2(40%)	2(40%)	1(20%)	0(%)	1(50%)	0(0%)	22.9%	34.8%	25.7%
TE	2(28.6%)	3(42.9%)	2(28.6%)	1(20%)	3(60%)	1(20%)	0(%)	0(%)	0(0%)	16.2%	34.3%	16.2%
Aver. (%)	57.14%	24.3%	18.6%	52%	34%	12%	20%	10%	15%

Abbreviations: S, sensitive; I, intermediate; R, resistance Anti; used, Antimicrobials used; No, Number; Env‘t, Environment; Aver., Average; for abbreviated drug name, see the abbreviation page.

Figure 1 Bar-chart showing the susceptibility patterns of E. coli O157:H7 from different sample types.

Figure 2 Conventional PCR products of hylA gene with an amplicon size of 1151 bp run on the gel electrophoresis for E.coli O157:H7 isolates.

Notes: M = 100 bp DNA marker, lane=2 and 4 are positive result for hylA gene, lane =6 and 7 negative and positive control for hylA gene respectively.

Figure 3 Conventional PCR products of stx1 gene with an amplicon size of 349 bp run on gel electrophoresis for E.coli O157:H7 isolates.

Notes: M= 100 bp DNA marker, lane=2, 3 and 4 positive results for stx1 gene, lane =6 and 7 negative and positive controls for stx1 gene respectively.