Total T Cell Density and Expression of T Memory Stem Cell Markers are Associated with Better Prognosis in Colon Cancer

Figures & data

Table 1 Patients’ Clinic-Pathological Characteristics

Clinic-Pathological Characteristics	Case	Proportion
Sex
Female	71	45.81%
Male	84	54.19%
Age, y
>60	64	41.29%
≥60	91	58.71%
Site of primary tumor
Left	60	38.71%
Right	95	61.29%
Tumor differentiation grade
Grade 1 or 2	85	54.84%
Grade 3	70	45.16%
Tumor size (pT)
1 or 2	12	7.74%
3 or 4	143	92.26%
Lymph node metastasis (pN)
N0	83	53.55%
N1-2	72	46.45%
Stage of disease
Stage I–II	83	53.55%
Stage III	72	46.45%
Follow-up data of patients
Alive	96	61.94%
Died	59	38.06%

Table 2 Associations Between Patients’ Characteristics and T Cell Density, CD27 and CD95 Expression

Clinic-Pathological Characteristics	T Cell Density		P value	CD27		P value	CD95		P value
	High	Low		−	+		−	+
Sex
Female	33	38	0.655	38	33	0.461	43	28	0.864
Male	44	40		46	38		52	32
Age, y
<60	33	31	0.181	31	33	0.054	39	25	0.203
≥60	44	47		53	38		56	35
Site of tumor
Left	30	30	0.363	35	25	0.411	39	21	0.451
Right	47	48		49	46		56	39
Differentiation grade
Grade 1 or 2	40	45	0.576	45	40	0.730	50	35	0.487
Grade 3	37	33		39	31		45	25
Lymph node metastasis (pN)
N0	47	38	0.285	50	35	0.202	51	34	0.716
N1-2	30	40		34	36		44	26
Stage of disease
Stage I–II	52	31	0.001	36	47	0.004	43	40	0.009
Stage III	25	47		48	24		52	20

Figure 1 Representative IHC staining of CD3, CD8, CD27, and CD95 in the core of the tumor (CT) and in the tumor’s invasive margin (IM) in colon tumor tissue and in normal colon tissue (100×).

Figure 2 Representative IHC staining of CD3, CD8, and CD27 in the same place at the same time, namely in the core of the tumor (CT) and in the tumor’s invasive margin (IM) in colon tumor tissue (100×).

Figure 3 Kaplan–Meier curves according to T cell density, CD27, and CD95 expression and Log rank test. (A) T cell density, n (low) = 80, n (high) = 75; (B) CD27 expression, n (low) = 62, n (high) = 93; (C) CD95 expression, n (low) = 56, n (high) = 99.

Table 3 Univariate and Multivariate Cox Regression Analysis of Survival Factors in Colon Cancer

Variables	Univariate Analysis			Multivariate Analysis
	HR	95% CI	P value	HR	95% CI	P value
Age (≥60 vs < 60), y	1.199	0.6825–2.098	0.525
Sex (male vs female)	1.290	0.767–2.169	0.336
Site of tumor (left vs right)	0.750	0.443–1.269	0.283
Differentiation grade (Grade 1 or 2 vs grade 3)	0.897	0.530–1.518	0.687
Lymph node metastasis (0 vs 1 or 2)	0.815	0.512–1.543	0.423
Stage of disease (I–II vs III)	0.227	0.127–0.407	<0.001	0.324	0.215–0.426	0.001
T cell density (high vs low)	0.387	0.223–0.672	0.001	0.423	0.305–0.908	0.021
CD27 expression (Positive vs negative)	0.321	0.317–0.922	0.024	0.325	0.217–0.653	0.025
CD95 expression (Positive vs negative)	0.422	0.227–0.784	0.006	0.451	0.229–0.714	0.036