Use of Silver Nanomaterials for Caries Prevention: A Concise Review

Figures & data

Figure 1 Flow chart of the literature search.

Table 1 Publications of Silver Nanomaterials and Cariogenic Bacteria (n = 59)

Nanomaterial	Study Design, Bacteria	Main Finding	Author, Year
AgNPs	In vitro study, S. mutans	MIC: 66.9±0 µg/mL	Espinosa-Cristóbal et al, 2009^Citation31
AgNPs	In vitro study, S. mutans	MIC: 141.2±45 µg/mL	Espinosa-Cristóbal et al, 2012^Citation47
AgNPs	In vitro study, S. mutans	MIC: 3.3±1.4 µg/mL MBC: 2.4 µg/mL	Santos et al, 2017^Citation32
AgNPs	In vitro study, S. mutans	MIC: 19.1 ppm	Fernandes et al, 2018^Citation33
AgNPs	In vitro study, S. mutans	Inhibited bacteria growth	Lu et al, 2013^Citation48
AgNPs	In vitro study, S. mutans	Inhibited bacteria growth	Emmanuel et al, 2015^Citation56
AgNPs	In vitro study, S. mutans	Inhibited bacterial growth	Wang et al, 2017^Citation57
AgNPs	In vitro study, S. mutans	Inhibited bacteria growth	Scarpelli et al, 2017^Citation41
AgNPs	In vitro study, S. mutans	Inhibited bacteria growth	Kumar et al, 2018^Citation58
AgNPs	In vitro study, S. mutans	Inhibited bacteria growth	Jesse et al, 2018^Citation40
AgNPs	In vitro study, S. mutans	Inhibited bacteria growth	Ahmed et al, 2019^Citation15
AgNPs	In vitro study, S. mutans	Inhibited bacteria growth and biofilm adhesion	Pérez-Díaz et al, 2015^Citation49
AgNPs	In vitro study, S. mutans	Inhibited bacteria growth and biofilm adhesion	Tavaf et al, 2017^Citation59
AgNPs	In vitro study, S. mutans	Inhibited bacteria growth and biofilm adhesion	Espinosa-Cristóbal et al, 2018^Citation38
AgNPs	In vitro study, S. mutans	Inhibited bacteria growth and biofilm adhesion	Schwass et al, 2018^Citation34
AgNPs	In vitro study, S. mutans	Inhibited biofilm adhesion	Espinosa-Cristóbal et al, 2013^Citation51
AgNPs	In vitro study, S. mutans	Inhibited biofilm adhesion	Besinis et al, 2014^Citation60
AgNPs	In vitro study, S. mutans	Inhibited biofilm adhesion	Freire et al, 2015^Citation36
AgNPs	In vitro study, S. mutans	Inhibited biofilm adhesion	Saafan et al, 2018^Citation61
AgNPs	Rat study, oral microcosm	Inhibited biofilm adhesion	Metin-Gursoy et al, 2017^Citation13
NSF	In vitro study, S. mutans	MIC: 33.5±14.5 μg/mL MBC: 50.3 μg/mL	Targino et al, 2014^Citation22
NSF	In vitro study, S. mutans	Inhibited biofilm adhesion	Silva et al, 2018^Citation18
NSF	In vitro study, S. mutans	Inhibited biofilm adhesion	Teixeira et al, 2018^Citation19
Resin with AgNPs	In vitro study, S. mutans	MIC: 6.1 µg/mL MBC: 6.1 µg/mL	Hernandez et al, 2010^Citation62
Resin with AgNPs	In vitro study, S. mutans	Inhibited bacteria growth	Ahn et al, 2009^Citation63
Resin with AgNPs	In vitro study, S. mutans	Inhibited bacteria growth	Fan et al, 2011^Citation64
Resin with AgNPs	In vitro study, S. mutans	Inhibited bacteria growth	Azarsina et al, 2013^Citation65
Resin with AgNPs	In vitro study, S. mutans	Inhibited bacteria growth	Kasraei et al, 2014^Citation66
Resin with AgNPs	In vitro study, S. mutans	Inhibited bacteria growth	Hernandez-Gomor et al, 2017^Citation25
Resin with AgNPs	In vitro study, S. mutans	Inhibited bacteria growth	Mazumder et al, 2018^Citation1
Resin with AgNPs	In vitro study, S. mutans	Inhibited bacteria growth	Mohammed et al, 2019^Citation67
Resin with AgNPs	In vitro study, S. mutans	Inhibited bacteria growth and biofilm adhesion	Cheng et al, 2013^Citation68
Resin with AgNPs	In vitro study, S. mutans	Inhibited biofilm adhesion	Ionescu et al, 2015^Citation69
Resin with AgNPs	In vitro study, S. mutans	Inhibited biofilm adhesion	Melo et al, 2016^Citation70
Resin with AgNPs	In vitro study, S. mutans	Inhibited biofilm adhesion	Cheng et al, 2012^Citation71
Resin with AgNPs	In vitro study, S. mutans	Inhibited biofilm adhesion	Cheng et al, 2012^Citation72
Resin with AgNPs	In vitro study, S. mutans	Inhibited biofilm adhesion	Li et al, 2013^Citation37
Resin with AgNPs	In vitro study, oral microcosm	Inhibited bacteria growth and biofilm adhesion	Cheng et al, 2012^Citation73
Resin with AgNPs	In vitro study, oral microcosm	Inhibited bacteria growth and biofilm adhesion	Zhang et al, 2013^Citation74
Resin with AgNPs	In vitro study, oral microcosm	Inhibited bacteria growth and biofilm adhesion	Cheng et al, 2013^Citation75
Resin with AgNPs	In vitro study, oral microcosm	Inhibited bacteria growth and biofilm adhesion	Sodagar et al, 2016^Citation42
Resin with AgNPs	In vitro study, oral microcosm	Inhibited biofilm adhesion	Zhang et al, 2012^Citation76
Resin with AgNPs	In vitro study, oral microcosm	Inhibited biofilm adhesion	Melo et al, 2013^Citation77
Resin with AgNPs	In vitro study, oral microcosm	Inhibited biofilm adhesion	Melo et al, 2013^Citation78
Resin with AgNPs	In vitro study, oral microcosm	Inhibited biofilm adhesion	Zhang et al, 2013^Citation24
Resin with AgNPs	In vitro study, oral microcosm	Inhibited biofilm adhesion	Zhang et al, 2013^Citation79
Resin with AgNPs	In vitro study, oral microcosm	Inhibited biofilm adhesion	Li et al, 2014^Citation80
Resin with AgNPs	In vitro study, oral microcosm	Inhibited biofilm adhesion	Cheng et al, 2016^Citation43
Resin with AgNPs	In vitro study, oral microcosm	Inhibited biofilm adhesion	Cao et al, 2017^Citation81
Resin with AgNPs	In vitro study, oral microcosm	Inhibited biofilm adhesion	Dias et al, 2019^Citation46
Resin with AgNPs	Clinical trial, oral microcosm	Inhibited bacteria growth and biofilm adhesion	Ghorbanzadeh et al, 2015^Citation28
Resin with AgNPs	Clinical trial, oral microcosm	Inhibited biofilm adhesion	Farhadian et al, 2016^Citation29
GIC with AgNPs	In vitro study, S. mutans	Inhibited bacteria growth	Li et al, 2013^Citation82
GIC with AgNPs	In vitro study, S. mutans	Inhibited bacteria growth and biofilm adhesion	El-Wassefy et al, 2018^Citation27
GIC with AgNPs	In vitro study, oral microcosm	Inhibited biofilm adhesion	Wang et al, 2015^Citation39
GIC with AgNPs	In vitro study, oral microcosm	Inhibited biofilm adhesion	Zhang et al, 2015^Citation83
GIC with AgNPs	In vitro study, oral microcosm	Inhibited biofilm adhesion	Zhang et al, 2016^Citation35
GIC with AgNPs	In vitro study, oral microcosm	Inhibited biofilm adhesion	Zhang et al, 2016^Citation44
GIC with AgNPs	Rat study, oral microcosm	Inhibited biofilm adhesion	Li et al, 2015^Citation30

Abbreviations: AgNPs, silver nanoparticles; S. mutans, Streptococcus mutans; MIC, minimum inhibitory concentration; MBC, minimum bactericidal concentration; NSF, nano silver fluoride; GIC, glass ionomer cement.

Table 2 Publications of Silver Nanomaterials and Mineral Content of Teeth (n = 11)

Nanomaterial	Study Design	Main Finding	Author, Year
AgNPs	In vitro study, enamel caries Group 1: AgNPs, Group 2: SDF, Group 3: Water	Mineral content SDF > AgNPs > Water	Scarpelli et al, 2017^Citation41
AgNPs	In vitro study, Sound enamel Group 1: AgNPs, Group 2: NaF, Group 3: Water	Mineral content NaF > AgNPs > Water	Wu et al, 2018^Citation23
AgNPs	Rat study, incisors Group 1: Bracket (AgNPs), Group 2: Bracket	Caries rate Bracket (AgNPs) < Bracket	Metin-Gursoy et al, 2017^Citation13
Resin with AgNPs	In vitro study, dentin caries Group 1: Resin (AgNPs), Group 2:Water	Mineral content Resin (AgNPs) > Water	Xiao et al, 2017^Citation14
Resin with AgNPs	Clinical trial (6 months), First molars, 40 children Group 1: Resin (AgNPs), Group2: Resin	Mineral content Resin (AgNPs) > Resin	Salas-López et al, 2017^Citation26
NSF	In vitro study, sound enamel Group 1: NSF, Group 2: NaF, Group 3: Water	Mineral content NSF, NaF > Water	Silva et al, 2018^Citation18
NSF	In vitro study, sound enamel Group 1: NSF, Group 2: NaF, Group 3: Water	Mineral content NSF, NaF > Water	Teixeira et al, 2018^Citation19
NSF	In vitro study, enamel caries Group 1: NSF, Group 2: NaF, Group 3: Water	Mineral content NSF > NaF > Water	Nozari et al, 2017^Citation20
NSF	In vitro study, enamel caries Group 1: NSF, Group 2: NaF, Group 3: Water	Mineral content NSF, NaF > Water	Silva et al, 2019^Citation16
NSF	Clinical trial, 1 year, 60 children 130 caries teeth Group 1: NSF, Group 2: Water	Arrested caries rate: NSF > Water	Santos et al, 2014^Citation21
NSF	Clinical trial, 1 year, 50 children, 159 caries teeth Group 1: NSF, Group 2: SDF	Arrested caries rate: No significant difference	Tirupathi et al, 2019^Citation17

Abbreviations: AgNPs, silver nanoparticles; SDF, silver diamine fluoride; NaF, sodium fluoride; NSF, nano silver fluoride.

Espinosa-Cristóbal LF, Martínez-Castañón GA, Martínez-Martínez RE, et al. Antibacterial effect of silver nanoparticles against Streptococcus mutans. Mater Lett. 2009;63(29):2603–2606. doi:10.1016/j.matlet.2009.09.018

 Web of Science ®Google Scholar

Espinosa-Cristóbal LF, Martinez-Castanon GA, Martínez-Martínez RE, et al. Antimicrobial sensibility of Streptococcus mutans serotypes to silver nanoparticles. Mater Sci Eng C. 2012;32(4):896–901. doi:10.1016/j.msec.2012.02.009

 Web of Science ®Google Scholar

Dos Santos Junior VE, Targino AGR, Flores MAP, et al. Antimicrobial activity of silver nanoparticle colloids of different sizes and shapes against Streptococcus mutans. Res Chem Intermed. 2017;43(10):5889–5899. doi:10.1007/s11164-017-2969-5

 Web of Science ®Google Scholar

Fernandes GL, Delbem ACB, Do Amaral JG, et al. Nanosynthesis of silver-calcium glycerophosphate: promising association against oral pathogens. Antibiotics. 2018;7(3). doi:10.3390/antibiotics7030077

 Google Scholar

Lu Z, Rong K, Li J, Yang H, Chen R. Size-dependent antibacterial activities of silver nanoparticles against oral anaerobic pathogenic bacteria. J Mater Sci Mater Med. 2013;24(6):1465–1471. doi:10.1007/s10856-013-4894-523440430

 PubMed Web of Science ®Google Scholar

Emmanuel R, Palanisamy S, Chen SM, et al. Antimicrobial efficacy of green synthesized drug blended silver nanoparticles against dental caries and periodontal disease causing microorganisms. Mater Sci Eng C Mater Biol Appl. 2015;56:374–379. doi:10.1016/j.msec.2015.06.03326249603

 PubMed Web of Science ®Google Scholar

Wang S, Wu J, Yang H, Liu X, Huang Q, Lu Z. Antibacterial activity and mechanism of Ag/ZnO nanocomposite against anaerobic oral pathogen Streptococcus mutans. J Mater Sci Mater Med. 2017;28(1):23. doi:10.1007/s10856-016-5837-828044252

 PubMed Web of Science ®Google Scholar

Scarpelli BB, Punhagui MF, Hoeppner MG, et al. In vitro evaluation of the remineralizing potential and antimicrobial activity of a cariostatic agent with silver nanoparticles. Braz Dent J. 2017;28(6):738–743. doi:10.1590/0103-644020170136529211131

 PubMedGoogle Scholar

Kumar A, Majumdar RS, Dhewa T. In vitro efficacy of biosynthesized AgNPs against Streptococcus mutans causing dental plaque formation. J Sci Ind Res. 2018;77(4):225–228.

 Web of Science ®Google Scholar

Jesse JT, Shobini J. A plausible antibacterial green synthesized AgNPs from Tridax procumbens leaf-flower extract. J Pure Appl Microbiol. 2018;12(4):2135–2142. doi:10.22207/JPAM.12.4.51

 Web of Science ®Google Scholar

Ahmed F, Prashanth ST, Sindhu K, Nayak A, Chaturvedi S. Antimicrobial efficacy of nanosilver and chitosan against Streptococcus mutans, as an ingredient of toothpaste formulation: an in vitro study. J Indian Soc Pedod Prev Dent. 2019;37(1):46–54. doi:10.4103/JISPPD.JISPPD_239_1830804307

 PubMedGoogle Scholar

Pérez-Díaz MA, Boegli L, James G, et al. Silver nanoparticles with antimicrobial activities against Streptococcus mutans and their cytotoxic effect. Mater Sci Eng C. 2015;55:360–366. doi:10.1016/j.msec.2015.05.036

 PubMed Web of Science ®Google Scholar

Tavaf Z, Tabatabaei M, Khalafi-Nezhad A, Panahi F. Evaluation of antibacterial, antibofilm and antioxidant activities of synthesized silver nanoparticles (AgNPs) and casein peptide fragments against Streptococcus mutans. Eur J Integr Med. 2017;12:163–171. doi:10.1016/j.eujim.2017.05.011

 Web of Science ®Google Scholar

Espinosa-Cristóbal LF, López-Ruiz N, Cabada-Tarín D, et al. Antiadherence and antimicrobial properties of silver nanoparticles against Streptococcus mutans on brackets and wires used for orthodontic treatments. J Nanomater. 2018;2018:1–11. doi:10.1155/2018/9248527

 Web of Science ®Google Scholar

Schwass DR, Lyons KM, Love R, Tompkins GR, Meledandri CJ. Antimicrobial activity of a colloidal AgNP suspension demonstrated in vitro against monoculture biofilms: toward a novel tooth disinfectant for treating dental caries. Adv Dent Res. 2018;29(1):117–123. doi:10.1177/002203451773649529355416

 PubMedGoogle Scholar

Espinosa-Cristóbal L, Martínez-Castañón G, Téllez-Déctor E, Niño-Martínez N, Zavala-Alonso N, Loyola-Rodríguez J. Adherence inhibition of Streptococcus mutans on dental enamel surface using silver nanoparticles. Mater Sci Eng C. 2013;33(4):2197–2202. doi:10.1016/j.msec.2013.01.039

 PubMed Web of Science ®Google Scholar

Besinis A, De Peralta T, Handy RD. Inhibition of biofilm formation and antibacterial properties of a silver nano-coating on human dentine. Nanotoxicology. 2014;8(7):745–754. doi:10.3109/17435390.2013.82534323875717

 PubMed Web of Science ®Google Scholar

Freire PL, Stamford TC, Albuquerque AJ, et al. Action of silver nanoparticles towards biological systems: cytotoxicity evaluation using hen’s egg test and inhibition of Streptococcus mutans biofilm formation. Int J Antimicrob Agents. 2015;45(2):183–187. doi:10.1016/j.ijantimicag.2014.09.00725455849

 PubMed Web of Science ®Google Scholar

Saafan A, Zaazou MH, Sallam MK, Mosallam O, El Danaf HA. Assessment of photodynamic therapy and nanoparticles effects on caries models. Open Access Maced J Med Sci. 2018;6(7):1289–1295. doi:10.3889/oamjms.2018.24130087739

 PubMedGoogle Scholar

Metin-Gursoy G, Taner L, Akca G. Nanosilver coated orthodontic brackets: in vivo antibacterial properties and ion release. Eur J Orthod. 2017;39(1):9–16. doi:10.1093/ejo/cjv09726787659

 PubMed Web of Science ®Google Scholar

Targino AGR, Flores MAP, Dos Santos Junior VE, et al. An innovative approach to treating dental decay in children. A new anti-caries agent. J Mater Sci Mater Med. 2014;25(8):2041–2047. doi:10.1007/s10856-014-5221-524818873

 PubMed Web of Science ®Google Scholar

Vieira Costa e Silva A, Teixeira JA, Mota CCBO, et al. In vitro morphological, optical and microbiological evaluation of nanosilver fluoride in the remineralization of deciduous teeth enamel. Nanotechnol Rev. 2018;7(6):509–520. doi:10.1515/ntrev-2018-0083

 Web of Science ®Google Scholar

Teixeira JA, Silva AVCE, Dos Santos Júnior VE, et al. Effects of a new nano-silver fluoride-containing dentifrice on demineralization of enamel and streptococcus mutans adhesion and acidogenicity. Int J Dent. 2018;2018:1351925. doi:10.1155/2018/135192529853891

 PubMed Web of Science ®Google Scholar

Hernandez-Sierra JF, Salas-Lopez EK, Martinez-Gutierrez F, et al. Bactericidal capacity of silver nanoparticles associated with Gantrez S-97 on Streptococcus mutans. J Clin Pediatr Dent. 2010;35(2):183–185. doi:10.17796/jcpd.35.2.c61l421mj0655lgm21417121

 PubMed Web of Science ®Google Scholar

Ahn S-J, Lee S-J, Kook J-K, Lim B-S. Experimental antimicrobial orthodontic adhesives using nanofillers and silver nanoparticles. Dent Mater. 2009;25(2):206–213. doi:10.1016/j.dental.2008.06.00218632145

 PubMed Web of Science ®Google Scholar

Fan C, Chu L, Rawls HR, Norling BK, Cardenas HL, Whang K. Development of an antimicrobial resin–a pilot study. Dent Mater. 2011;27(4):322–328. doi:10.1016/j.dental.2010.11.00821112619

 PubMed Web of Science ®Google Scholar

Azarsina M, Kasraei S, Yousefi-Mashouf R, Dehghani N, Shirinzad M. The antibacterial properties of composite resin containing nanosilver against Streptococcus mutans and Lactobacillus. J Contemp Dent Pract. 2013;14(6):1014–1018. doi:10.5005/jp-journals-10024-144224858742

 PubMedGoogle Scholar

Kasraei S, Sami L, Hendi S, Alikhani MY, Rezaei-Soufi L, Khamverdi Z. Antibacterial properties of composite resins incorporating silver and zinc oxide nanoparticles on Streptococcus mutans and Lactobacillus. Restor Dent Endod. 2014;39(2):109–114.24790923

 PubMedGoogle Scholar

Hernandez-Gomora AE, Lara-Carrillo E, Robles-Navarro JB, et al. Biosynthesis of silver nanoparticles on orthodontic elastomeric modules: evaluation of mechanical and antibacterial properties. Molecules. 2017;22(9):1407. doi:10.3390/molecules22091407

 PubMed Web of Science ®Google Scholar

Mazumder JA, Khatoon N, Batra P, Sardar M. Biosynthesized silver nanoparticles for orthodontic applications. Adv Sci Eng Med. 2018;10(12):1169–1173. doi:10.1166/asem.2018.2289

 Google Scholar

Mohammed HF, Riad MI. The effect of silver nanoparticles incorporation in the self-etch adhesive system on its antibacterial activity and degree of conversion: an in-vitro study [version 1; peer review: awaiting peer review]. F1000Research. 2019;8:244. doi:10.12688/f1000research.17687.1

 Google Scholar

Cheng L, Zhang K, Weir MD, Liu H, Zhou X, Xu HHK. Effects of antibacterial primers with quaternary ammonium and nano-silver on Streptococcus mutans impregnated in human dentin blocks. Dent Mater. 2013;29(4):462–472. doi:10.1016/j.dental.2013.01.01123422420

 PubMed Web of Science ®Google Scholar

Ionescu AC, Brambilla E, Travan A, et al. Silver-polysaccharide antimicrobial nanocomposite coating for methacrylic surfaces reduces Streptococcus mutans biofilm formation in vitro. J Dent. 2015;43(12):1483–1490. doi:10.1016/j.jdent.2015.10.00626477347

 PubMed Web of Science ®Google Scholar

Melo MA, Orrego S, Weir MD, Xu HH, Arola DD. Designing multiagent dental materials for enhanced resistance to biofilm damage at the bonded interface. ACS Appl Mater Interfaces. 2016;8(18):11779–11787. doi:10.1021/acsami.6b0192327081913

 PubMed Web of Science ®Google Scholar

Cheng L, Weir MD, Xu HHK, et al. Antibacterial amorphous calcium phosphate nanocomposites with a quaternary ammonium dimethacrylate and silver nanoparticles. Dent Mater. 2012;28(5):561–572. doi:10.1016/j.dental.2012.01.00522305716

 PubMed Web of Science ®Google Scholar

Cheng L, Zhang K, Melo MAS, Weir MD, Zhou X, Xu HHK. Anti-biofilm dentin primer with quaternary ammonium and silver nanoparticles. J Dent Res. 2012;91(6):598–604. doi:10.1177/002203451244412822492276

 PubMed Web of Science ®Google Scholar

Li F, Weir MD, Chen J, Xu HHK. Comparison of quaternary ammonium-containing with nano-silver-containing adhesive in antibacterial properties and cytotoxicity. Dent Mater. 2013;29(4):450–461. doi:10.1016/j.dental.2013.01.01223428077

 PubMed Web of Science ®Google Scholar

Cheng L, Weir MD, Xu HH, et al. Effect of amorphous calcium phosphate and silver nanocomposites on dental plaque microcosm biofilms. J Biomed Mater Res B Appl Biomater. 2012;100(5):1378–1386. doi:10.1002/jbm.b.3270922566464

 PubMedGoogle Scholar

Zhang K, Cheng L, Imazato S, et al. Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm, cytotoxicity and dentine bond properties. J Dent. 2013;41(5):464–474. doi:10.1016/j.jdent.2013.02.00123402889

 PubMed Web of Science ®Google Scholar

Cheng L, Weir MD, Zhang K, Arola DD, Zhou X, Xu HHK. Dental primer and adhesive containing a new antibacterial quaternary ammonium monomer dimethylaminododecyl methacrylate. J Dent. 2013;41(4):345–355. doi:10.1016/j.jdent.2013.01.00423353068

 PubMed Web of Science ®Google Scholar

Sodagar A, Akhavan A, Hashemi E, et al. Evaluation of the antibacterial activity of a conventional orthodontic composite containing silver/hydroxyapatite nanoparticles. Prog Orthod. 2016;17(1):40. doi:10.1186/s40510-016-0153-x27819127

 PubMedGoogle Scholar

Zhang K, Melo MAS, Cheng L, Weir MD, Bai Y, Xu HHK. Effect of quaternary ammonium and silver nanoparticle-containing adhesives on dentin bond strength and dental plaque microcosm biofilms. Dent Mater. 2012;28(8):842–852. doi:10.1016/j.dental.2012.04.02722592165

 PubMed Web of Science ®Google Scholar

Melo MAS, Cheng L, Zhang K, Weir MD, Rodrigues LKA, Xu HHK. Novel dental adhesives containing nanoparticles of silver and amorphous calcium phosphate. Dent Mater. 2013;29(2):199–210. doi:10.1016/j.dental.2012.10.00523138046

 PubMed Web of Science ®Google Scholar

Melo MA, Cheng L, Weir MD, Hsia RC, Rodrigues LK, Xu HH. Novel dental adhesive containing antibacterial agents and calcium phosphate nanoparticles. J Biomed Mater Res B Appl Biomater. 2013;101(4):620–629. doi:10.1002/jbm.b.3286423281264

 PubMedGoogle Scholar

Zhang K, Li F, Imazato S, et al. Dual antibacterial agents of nano-silver and 12-methacryloyloxydodecylpyridinium bromide in dental adhesive to inhibit caries. J Biomed Mater Res B Appl Biomater. 2013;101(6):929–938. doi:10.1002/jbm.b.3289823529901

 PubMedGoogle Scholar

Zhang K, Cheng L, Wu EJ, Weir MD, Bai Y, Xu HHK. Effect of water-ageing on dentine bond strength and anti-biofilm activity of bonding agent containing new monomer dimethylaminododecyl methacrylate. J Dent. 2013;41(6):504–513. doi:10.1016/j.jdent.2013.03.01123583528

 PubMed Web of Science ®Google Scholar

Li F, Weir MD, Fouad AF, Xu HHK. Effect of salivary pellicle on antibacterial activity of novel antibacterial dental adhesives using a dental plaque microcosm biofilm model. Dent Mater. 2014;30(2):182–191. doi:10.1016/j.dental.2013.11.00424332270

 PubMed Web of Science ®Google Scholar

Cheng L, Zhang K, Zhou CC, Weir MD, Zhou XD, Xu HH. One-year water-ageing of calcium phosphate composite containing nano-silver and quaternary ammonium to inhibit biofilms. Int J Oral Sci. 2016;8(3):172–181. doi:10.1038/ijos.2016.1327281037

 PubMed Web of Science ®Google Scholar

Cao W, Zhang Y, Wang X, et al. Development of a novel resin-based dental material with dual biocidal modes and sustained release of Ag(+) ions based on photocurable core-shell AgBr/cationic polymer nanocomposites. J Mater Sci Mater Med. 2017;28(7):103. doi:10.1007/s10856-017-5918-328534286

 PubMed Web of Science ®Google Scholar

Dias HB, Bernardi MIB, Marangoni VS, de Abreu Bernardi AC, de Souza Rastelli AN, Hernandes AC. Synthesis, characterization and application of Ag doped ZnO nanoparticles in a composite resin. Mater Sci Eng C Mater Biol Appl. 2019;96:391–401. doi:10.1016/j.msec.2018.10.06330606547

 PubMed Web of Science ®Google Scholar

Ghorbanzadeh R, Pourakbari B, Bahador A. Effects of baseplates of orthodontic appliances with in situ generated silver nanoparticles on cariogenic bacteria: a randomized, double-blind cross-over clinical trial. J Contemp Dent Pract. 2015;16(4):291. doi:10.5005/jp-journals-10024-167826067732

 PubMedGoogle Scholar

Farhadian N, Usefi Mashoof R, Khanizadeh S, Ghaderi E, Farhadian M, Miresmaeili A. Streptococcus mutans counts in patients wearing removable retainers with silver nanoparticles vs those wearing conventional retainers: a randomized clinical trial. Am J Orthod Dentofacial Orthop. 2016;149(2):155–160. doi:10.1016/j.ajodo.2015.07.03126827971

 PubMed Web of Science ®Google Scholar

Li F, Li Z, Liu G, He H. Long-term antibacterial properties and bond strength of experimental nano silver-containing orthodontic cements. J. Wuhan Univ Technol Mater Sci Ed. 2013;28(4):849–855. doi:10.1007/s11595-013-0781-7

 Web of Science ®Google Scholar

El-Wassefy NA, El-Mahdy RH, El-Kholany NR. The impact of silver nanoparticles integration on biofilm formation and mechanical properties of glass ionomer cement. J Esthet Restor Dent. 2018;30(2):146–152. doi:10.1111/jerd.1235329197139

 PubMed Web of Science ®Google Scholar

Wang X, Wang B, Wang Y. Antibacterial orthodontic cement to combat biofilm and white spot lesions. Am J Orthod Dentofacial Orthop. 2015;148(6):974–981. doi:10.1016/j.ajodo.2015.06.01726672703

 PubMed Web of Science ®Google Scholar

Zhang N, Chen C, Weir MD, Bai Y, Xu HHK. Antibacterial and protein-repellent orthodontic cement to combat biofilms and white spot lesions. J Dent. 2015;43(12):1529–1538. doi:10.1016/j.jdent.2015.09.00626427311

 PubMed Web of Science ®Google Scholar

Zhang N, Weir MD, Chen C, Melo MAS, Bai Y, Xu HHK. Orthodontic cement with protein-repellent and antibacterial properties and the release of calcium and phosphate ions. J Dent. 2016;50:51–59. doi:10.1016/j.jdent.2016.05.00127157089

 PubMed Web of Science ®Google Scholar

Zhang N, Melo MAS, Antonucci JM, et al. Novel dental cement to combat biofilms and reduce acids for orthodontic applications to avoid enamel demineralization. Materials. 2016;9(6):413.

 PubMed Web of Science ®Google Scholar

Li F, Fang M, Peng Y, Zhang J. Antibacterial properties of nano silver-containing orthodontic cements in the rat caries disease model. J. Wuhan Univ Technol Mater Sci Ed. 2015;30(6):1291–1296. doi:10.1007/s11595-015-1310-7

 Web of Science ®Google Scholar

Wu R, Zhao Q, Lu S, Fu Y, Yu D, Zhao W. Inhibitory effect of reduced graphene oxide-silver nanocomposite on progression of artificial enamel caries. J Appl Oral Sci. 2018;27:e20180042. doi:10.1590/1678-7757-2018-004230540069

 PubMed Web of Science ®Google Scholar

Xiao S, Liang K, Weir MD, et al. Combining bioactive multifunctional dental composite with PAMAM for root dentin remineralization. Materials. 2017;10(1):89.

 PubMed Web of Science ®Google Scholar

Salas-Lopez EK, Pierdant-Perez M, Hernandez-Sierra JF, Ruiz F, Mandeville P, Pozos-Guillen AJ. Effect of silver nanoparticle-added pit and fissure sealant in the prevention of dental caries in children. J Clin Pediatr Dent. 2017;41(1):48–52. doi:10.17796/1053-4628-41.1.4828052214

 PubMed Web of Science ®Google Scholar

Nozari A, Ajami S, Rafiei A, Niazi E. Impact of nano hydroxyapatite, nano silver fluoride and sodium fluoride varnish on primary teeth enamel remineralization: an in vitro study. J Clin Diagn Res. 2017;11(9):Zc97–Zc100. doi:10.7860/JCDR/2017/30108.10694

 PubMedGoogle Scholar

Silva A, JdA T, Melo Júnior P, et al. Remineralizing potential of nano-silver-fluoride for tooth enamel: an optical coherence tomography analysis. Pesquisa Brasileira em Odontopediatria e Clínica Integrada. 2019;19(1):1–13.

 Web of Science ®Google Scholar

Dos Santos VE, Vasconcelos Filho A, Targino AGR, et al. A new “silver-bullet” to treat caries in children–nano silver fluoride: a randomised clinical trial. J Dent. 2014;42(8):945–951. doi:10.1016/j.jdent.2014.05.01724930870

 PubMed Web of Science ®Google Scholar

Tirupathi S, Svsg N, Rajasekhar S, Nuvvula S. Comparative cariostatic efficacy of a novel nano-silver fluoride varnish with 38% silver diamine fluoride varnish a double-blind randomized clinical trial. J Clin Exp Dent. 2019;e105–e112. doi:10.4317/jced.5499530805113

 PubMedGoogle Scholar