Figures & data
Figure 1 XRD of MAC (A), MAC microsphere (B), and BSA-loaded microspheres (C).
Abbreviations: BSA, bovine serum albumin; MAC, multi-(amino acid) copolymer; XRD, X-ray diffraction.
Figure 2 FTIR of MAC (A), MAC microsphere (B), and BSA-loaded (C) microspheres.
Abbreviations: BSA, bovine serum albumin; FTIR, Fourier transform infrared spectrometry; MAC, multi-(amino acid) copolymer.
Table 1 Effects of surfactant ratio of Tween 80 to Span 80 on the particle size and encapsulation efficiency of BSA-loaded microspheres
Figure 3 SEM images of morphology of BSA-loaded microspheres prepared with different surfactant ratios of Tween 80 to Span 80 of 3:2 (A and B), 7:3 (C), and 4:1 (D).
Abbreviations: BSA, bovine serum albumin; SEM, scanning electron microscopy.
Figure 4 SEM images of morphology of BSA-loaded microspheres with benzyl alcohol in the solidification step (A) and without benzyl alcohol in the external water phase in the emulsification step (B).
Abbreviations: BSA, bovine serum albumin; SEM, scanning electron microscopy.
Table 2 Effects of addition of benzyl alcohol on particle size and encapsulation efficiency of BSA-loaded microspheres
Table 3 Effects of ratio of surfactant concentration on particle size and encapsulation efficiency of BSA-loaded microspheres
Table 4 Effects of polymer concentration on particle size and encapsulation efficiency of BSA-loaded microspheres
Figure 5 Profiles of BSA release from microspheres prepared with different polymer concentrations into PBS over time.
Abbreviations: BSA, bovine serum albumin; PBS, phosphate buffered saline.
Figure 6 Weight loss of unloaded and BSA-loaded microspheres in PBS over time.
Abbreviations: BSA, bovine serum albumin; PBS, phosphate buffered saline.
Figure 7 Change of pH in PBS after immersion of unloaded microspheres and BSA-loaded microspheres over time.
Abbreviations: BSA, bovine serum albumin; PBS, phosphate buffered saline.
