The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Reduces Malondialdehyde and Nitric Oxide Levels in Deep Dermal Burn Injury

Figures & data

Figure 1 Histopathological view of the deep dermal burn model with hematoxylin-eosin staining shows the absence of the epidermal and dermal layers of the skin (HE staining, magnification 40x).

Table 1 Measurement Results for Malondialdehyde and Nitric Oxide Levels and p-values (One-Way ANOVA)

Time Points	Group of Intervention	Blood MDA Level	Tissue MDA Level	Blood NO Level	Tissue NO Level
8th hours	Treatment Group	0.550 (±0.113)	0.593 (±0.050)	15.333 (±3.055)	20.667 (±1.528)
	Positive Control	0.603 (±0.065)	0.687 (±0.029)	40.333 (±9.866)	35.000 (±3.605)
	Negative Control	0.813 (±0.078)	0.843 (±0.125)	48.333 (±4.619)	33.667 (±2.309)
	Normal Control	0.553 (±0.067)	0.657 (±0.100)	14.000 (±1.000)	18.333 (±1.528)
		p= 0.014*	p= 0.036*	p= 0.000*	p= 0.000*
24th hours	Treatment Group	0.537 (±0.185)	0.677 (±0.076)	19.333 (±4.509)	18.000 (±7.211)
	Positive Control	0.703 (±0.015)	0.730 (±0.061)	28.333 (±4.619)	16.667 (±6.028)
	Negative Control	0.820 (±0.171)	0.767 (±0.081)	36.000 (±1.000)	28.000 (±2.646)
	Normal Control	0.547 (±0.064)	0.650 (±0.096)	13.333 (±0.577)	18.667 (±1.528)
		p= 0.080	p= 0.339	p= 0.000*	p= 0.079
48th hours	Treatment Group	0.593 (±0.137)	0.570 (±0.656)	21.667 (±3.786)	3.333 (±3.519)
	Positive Control	0.880 (±0.072)	0.713 (±0.032)	24.667 (±2.517)	31.667 (±9.713)
	Negative Control	0.813 (±0.035)	1.523 (±0.256)	37.333 (±5.859)	51.667 (±6.110)
	Normal Control	0.550 (±0.069)	0.653 (±0.104)	15.000 (±1.000)	15.667 (±1.155)
		p= 0.004*	p= 0.041*	p= 0.001*	p= 0.000*

Note: *95% confidence interval.

Figure 2 Multiple comparisons of blood malondialdehyde levels. *p< 0.05.

Figure 3 Multiple comparisons of tissue malondialdehyde levels. *p< 0.05.

Figure 4 Multiple comparisons of blood nitric oxide levels. *p< 0.05.

Figure 5 Multiple comparisons of tissue nitric oxide levels. *p< 0.05.

Table 2 Regression Test Results in the Treatment Group

	P-value	R	R-Square	Equation
MDA Blood	0.022*	0.875	0.766	Y= 0.817 + (−0.257X)
MDA Tissue	0.002*	0.965	0.932	Y= 1.043 + (−0.427X)
NO Blood	0.000*	0.984	0.968	Y= 40.557 + (−21.78X)
NO Tissue	0.000*	0.991	0.982	Y= 37.777 + (−23.777X)

Note: *95% confidence interval.

Table 3 Regression Test Results in the Positive Control Group

	P-value	R	R-Square	Equation
MDA Blood	0.114	0.710	0.504	Y= 0.817 + (−0.090X)
MDA Tissue	0.004*	0.948	0.898	Y= 1.043 + (−0.333X)
NO Blood	0.028*	0.861	0.742	Y= 40.557 + (−9.447X)
NO Tissue	0.041*	0.830	0.689	Y= 37.777 + (−10X)

Note: *95% confidence interval.