Exercise training increases spatial memory via reducing contralateral hippocampal NMDAR subunits expression in intracerebral hemorrhage rats

Figures & data

Table 1 Modified Neurological Severity Score (mNSS) in rats

Scoring items	Score (points)	Scoring standard
Exercise test	1	Forelimb flexion (increasing tail in rats)
	1	Flexion of hind limbs (raising tail of rats)
	1	Head deviation from vertical axis >100 within 30 s (increase tail of rats)
	0	Walk normally (put rats on the floor)
	1	Can’t walk straight (put rats on the floor)
	2	Turn sideways toward paralysis (place rats on the floor)
	3	Tilt to the side of paralysis (place rats on the floor)
Sensory test	1	Placement test (deep feeling, pressing rat paws on the edge of the table to stimulate limb muscles)
	2	Proprioceptive Sensory Test (Visual and Tactile Test)
Balance beam test	0	Stable balance posture
	1	Grasp the edge of the balance beam
	2	Hold tight to the balance beam and drop one limb from the balance beam
	3	Holding the balance beam tightly, the limbs drop from the balance beam or rotate on the balance beam (>60 s)
	4	Trying to balance on the balance beam but falling (>40 s)
	5	Trying to balance on the balance beam but falling (>20 s)
	6	Falling; not attempting to balance on the balance beam (<20 s)
Absence of reflex and abnormal motion	1	Auricular reflex (shake your head when touching the external auditory canal)
	1	Corneal reflex (blinking when touching the cornea with cotton wool)
	1	Panic reflex (a motor response to the noise of fast-bouncing cardboard)
	1	Epilepsy epilepsy, myoclonus, dystonia

Figure 1 Exercise training improves the memory of intracerebral hemorrhage rats in the water maze test. (A) The mNSS score of each group after modeling. (B) Average speed of each group per day. (C) Average escape latency of each group per day in the place navigation task. (D) Percentage of time in target quadrant for each group in the spatial probe task. (E) Percentage of distance in target quadrant for each group in the spatial probe task. (F) Representative trace of spatial probe task. *P<0.05 vs. the MD group, ^#P<0.05 vs. the SM group.

Abbreviations: EX, exercise group; MD, model group; SM, sham operation group.

Figure 2 Exercise training reduces the level of NR1 and NR2B in the contralateralhippocampal CA3 region of intracerebral hemorrhage rats. (A) Representative image of the western blot. (B) Western blot analysis of NR1 expression. (C) Western blot analysis of NR2B expression. ^##P>0.05 vs. the SM group, ^*P<0.05 vs. the MD group, ^#P<0.05 vs. the SM group.

Abbreviations: EX, exercise group; MD, model group; SM, sham operation group.