Structure–Function Correlation Using OCT Angiography And Microperimetry In Diabetic Retinopathy

Figures & data

Figure 1 Real example of microperimetry performed for the study in a right eye. Microperimetric results of the right eye of a healthy subject. Retinal sensitivity is represented on the studied point in green color by a number and express in decibels.

Figure 2 Customized 13-points-grid for microperimetry for right and left eyes. Diagram showing the location of each studied point in the microperimetric exam. The microperimetry covers the 4.5×4.5 mm of the central macula. Note that in each area of the macula the grid is named equally to the same grid in the other eye in order to analyze them as the same topographic area.

Abbreviation: RS, retinal sensitivity.

Figure 3 Nine-sectors-grid for vessel density measured by OCT-A for right and left eyes. Diagram showing the location of each studied sector in the OCT-A. The exam covers the 4.5×4.5 mm of the central macula. Note that in each area of the macula the grid is named equally to the same grid in the other eye in order to analyze them as the same topographic area. Also, correlation between OCT-A and microperimetry are calculated following this grid.

Abbreviation: S, sector.

Table 1 Patients Demographics And Clinical Characteristics Of Diabetic Patients With Diabetic Retinopathy And Non-Diabetic Controls

Clinical Characteristics	Diabetics With DR	Non-Diabetic Controls	p-Values
N, eyes	25	25
Age, years, mean (SD)^a	51.88 (13.62)	43.48 (13.42)	0.039
Sex, n, female/male	15/10	11/14
BCVA, Snellen, mean (SD)^b	0.8 (0.21)	0.98 (0.05)	<0.001
Eye, n, right/left	14/11	13/12
Diabetic macular edema	No	–
Diabetic retinopathy severity
Mild, n (%)	3 (12%)	–
Moderate, n (%)	9 (36%)	–
Proliferative, n (%)	13 (52%)	–

Notes: ^aDiabetic group is older than control subjects (Mann–Whitney U-test). ^bBCVA is better in control group than diabetic group (Mann–Whitney U-test).

Abbreviations: DR, diabetic retinopathy; N, sample size; n, sample size; BCVA, best corrected visual acuity.

Table 2 Vessel Density In Superficial Capillary Plexus In The 9-Areas-Grid In Diabetic Retinopathy And Controls

Grid	Mean VD Superficial Plexus Diabetic Group (SD)		Mean VD Superficial Plexus Control Group (SD)		P-Value
S 1	19.84	(3.82)	20.12	(4.20)	0.807
S 2	21.28	(3.18)	24.16	(3.98)	0.004*
S 3	23.36	(3.12)	26.88	(5.22)	<0.001*
S 4	20.48	(3.92)	23.64	(4.89)	0.007*
S 5	14.96	(4.03)	19.12	(4.03)	0.002*
S 6	20.60	(6.02)	26.12	(3.63)	<0.001*
S 7	19.24	(3.99)	19.92	(5.05)	0.600
S 8	20.20	(5.38)	23.20	(4.49)	0.009*
S 9	22.76	(4.93)	26.68	(3.22)	<0.001*

Notes: Mean vessel density values in superficial plexus are shown in the two groups. Note that statistical significant differences are seen in nasal and central areas but not in S 1 and S 7 (temporal-superior and temporal-inferior areas). * Statistical significant differences (p<0.05).

Abbreviations: VD, vessel density; S, sector.

Table 3 Mean Vessel Density In Deep Capillary Plexus In The 9-Areas-Grid In Diabetic Retinopathy Group And Healthy Control Group

Grid	Mean VD Deep Plexus Diabetic Group (SD)		Mean VD Deep Plexus Control Group (SD)		P-Value
S 1	15.24	(4.37)	16.76	(3.84)	0.198
S 2	14.92	(5.04)	19.44	(3.45)	0.001*
S 3	13.52	(6.38)	18.20	(4.38)	0.004*
S 4	16.76	(4.61)	20.00	(3.34)	0.007*
S 5	7.72	(3.81)	13.28	(3.82)	<0.001*
S 6	14.76	(6.30)	22.64	(3.63)	<0.001*
S 7	13.72	(4.47)	14.24	(5.58)	0.718
S 8	13.16	(5.62)	16.76	(4.64)	0.017*
S 9	11.32	(4.85)	15.80	(4.89)	0.002*

Notes: Mean vessel density values in deep plexus are shown in the two groups. Statistical significant differences are remarkable in the nasal and central areas but not in S 1 and S 7 (temporal-superior and temporal-inferior sectors). * Statistical significant differences (p<0.05).

Abbreviations: VD, vessel density; D, deep plexus sector.

Table 4 Mean Retinal Sensitivity In Each Point Of The 13-Points-Grid In Diabetic Retinopathy Group And Control Group

Point	Mean RS Diabetic Group (SD)		Mean RS Control Group (SD)		P-Value
1	26.08	(5.92)	30.92	(2.72)	<0.001
2	28.36	(2.61)	32.00	(1.44)	<0.001
3	28.08	(2.31)	31.56	(2.10)	<0.001
4	27.76	(6.29)	32.72	(1.84)	<0.001
5	23.80	(6.58)	30.80	(2.77)	<0.001
6	29.96	(1.81)	32.48	(1.66)	<0.001
7	28.88	(1.90)	31.64	(1.82)	<0.001
8	28.56	(3.27)	31.44	(2.12)	<0.001
9	28.32	(3.20)	31.88	(1.76)	<0.001
10	26.96	(5.21)	31.88	(1.86)	<0.001
11	28.48	(2.96)	31.84	(2.23)	<0.001
12	27.04	(5.77)	31.76	(1.96)	<0.001
13	28.72	(4.50)	31.04	(4.48)	<0.001

Notes: In this table mean retinal sensitivity is represented for the DR patients and for control subjects. All the studied points prove statistical significant differences (p<0.05) between the two groups.