Protein Expression of Folate Receptor Alpha in Adenoid Cystic Carcinoma of the Head and Neck

Figures & data

Table 1 Baseline patient characteristics of adenoid cystic carcinoma patients

		Number of Patients	Percentage (%)
Sex	Female	23	53%
	Male	20	47%
T Stage	1	5	12%
	2	6	14%
	3	8	19%
	4	23	53%
	x	1	2%
N Stage	0	33	77%
	1	4	9%
	2	6	14%
M Stage	0	40	93%
	1	3	7%
Stage	I	5	12%
	II	7	16%
	III	5	12%
	IV	25	58%
	x	1	2%
Grading - Spiro	1	26	60%
	2	10	23%
	3	3	7%
	x	4	9%
Grading - Perzin/Szanto	1	7	16%
	2	22	51%
	3	10	23%
	x	4	9%
Localization	Minor	28	65%
	Major	15	35%
Perineural Invasion	No	21	49%
	Yes	22	51%
Lymphovascular Invasion	No	37	86%
	Yes	6	14%

Table 2 Correlation analysis for associations between expression of FRα and clinicopathological features

		FRα
		Negative	Positive	p value
Sex	Female	12 (52%)	11 (55%)
	Male	11 (48%)	9 (45%)	0.853
Age	≤59	10 (43%)	9 (45%)
	>59	13 (57%)	11 (55%)	0.92
T Stage	T1-2	5 (22%)	6 (32%)
	T3-4	18 (78%)	13 (68%)	0.504
N Stage	N0	16 (70%)	17 (85%)
	N1-2	7 (30%)	3 (15%)	0.294
M Stage	M0	20 (87%)	20 (100%)
	M1	3 (13%)	0 (0%)	0.236
Stage	I–II	5 (22%)	7 (37%)
	III–IV	18 (78%)	12 (63%)	0.323
Grading - Spiro	1	12 (57%)	14 (78%)
	2	7 (33%)	3 (17%)
	3	2 (%)	1 (%)	0.449
Grading - Perzin/Szanto	1	3 (14%)	4 (22%)
	2	11 (52%)	11 (61%)
	3	7 (33%)	3 (17%)	0.56
Localization	Minor	16 (70%)	12 (60%)
	Major	7 (30%)	8 (40%)	0.512
Perineural invasion	No	12 (52%)	9 (45%)
	Yes	11 (48%)	11 (55%)	0.639
Lymphovascular Invasion	No	19 (83%)	18 (90%)
	Yes	4 (17%)	2 (10%)	0.669

Note: Correlation was analyzed using Fisher’s exact or chi-squared test.

Figure 1 (A) Negative, (B) low positive, (C) moderately positive and (D) high positive staining of FRα in ACC.

Table 3 Univariable and multivariable analysis of cause-specific survival (CSS) and disease-free survival (DFS) and FRα expression

		Univariable			Multivariable
		HR	95% CI	p value	HR	95% CI	p value
CSS	FRα	0.39	0.12–1.22	0.105	0.48	0.14–1.67	0.247
	FRα score	0.66	0.34–1.28	0.221	0.68	0.35–1.31	0.249
DFS	FRα	0.91	0.38–2.15	0.826	1.04	0.41–2.63	0.932
	FRα score	1.23	0.81–1.88	0.332	1.83	0.80–2.03	0.305

Note: Multivariable analysis was corrected for staging and perineural invasion.

Abbreviations: HR, hazard ratio; 95% CI, 95% confidence interval.

Figure 2 Kaplan–Meier survival curves for (A) cause-specific survival (CSS) and (B) disease-free survival (DFS) and FRα protein expression. Survival was analyzed using log-rank test. p, log-rank p value.