Genetic Variants, Circulating Level of MCP1 with Risk of Chronic Obstructive Pulmonary Disease: A Case-Control Study

Figures & data

Table 1 Demographics of Study Participants

	COPD Cases (N=450)	Controls (N=500)	P-value
Age (years)
≤60	139 (30.9%)	153 (30.6%)	0.923
>60	311 (69.1%)	347 (69.4%)
Gender
Male	320 (71.1%)	354 (70.8%)	0.916
Female	130 (28.9%)	146 (29.2%)
BMI (kg/m^2)	23.3±3.11	23.6±3.14	0.140
Smoking status
Ever	270 (60.0%)	156 (31.2%)	<0.001
Never	180 (40.0%)	344 (68.8%)
MCP-1, pg/mL	175.6 (141.6–211.6)	157.1 (126.8–186.7)	<0.001
FEV1/FVC	0.55±0.09	0.81±0.10	<0.001
Gold grades
A and B	237 (52.7%)
C and D	213 (47.3%)

Note: Bold values are statistically significant (P<0.05).

Abbreviation: BMI, body mass index.

Table 2 Logistic Regression Analysis of Associations between MCP1 Gene Polymorphisms and Risk of COPD

Variants	Cases (n=450)	Controls (n=500)	OR (95% CI)*	P
rs1024611
AA	195	259	1.00 (reference)
AG	204	201	1.40 (1.05–1.87)	0.022
GG	51	40	1.76 (1.11–2.79)	0.016
G vs A			1.37 (1.11–1.69)	0.004
Dominant model			1.46 (1.11–1.92)	0.006
Recessive model			1.53 (0.97–2.41)	0.068
rs2857656
GG	216	241	1.00 (reference)
CG	207	221	1.09 (0.68–1.75)	0.731
CC	27	38	0.82 (0.54–1.25)	0.364
C vs G			1.01 (0.98–1.04)	0.726
Dominant model			1.05 (0.25–4.39)	0.948
Recessive model			0.81 (0.54–1.22)	0.306
rs4586
CC	132	192	1.00 (reference)
CT	235	230	1.55 (1.15–2.09)	0.004
TT	83	78	1.61 (1.09–2.39)	0.018
T vs C			1.33 (1.09–1.63)	0.006
Dominant model			1.56 (1.18–2.07)	0.002
Recessive model			1.27 (0.87–1.87)	0.221

Notes: *Adjusted for age, gender, BMI, and smoking status; bold values are statistically significant (P<0.05).

Table 3 Logistic Regression Analysis of Associations between MCP1 Gene Polymorphisms and Risk of COPD, Stratified by Gold Grades

	GOLD A and B		GOLD C and D
Variants	OR (95% CI)*	P	OR (95% CI)*	P
rs1024611
AA	1.00 (reference)		1.00 (reference)
AG	1.38 (0.99–1.91)	0.055	1.32 (0.94–1.84)	0.111
GG	1.94 (1.15–3.27)	0.012	1.43 (0.8–2.55)	0.222
G vs A	1.39 (1.1–1.76)	0.005	1.24 (0.97–1.58)	0.086
Dominant model	1.53 (1.1–2.12)	0.011	1.39 (0.98–1.97)	0.065
Recessive model	1.73 (1.04–2.89)	0.035	1.31 (0.71–2.43)	0.394
rs2857656
GG	1.00 (reference)		1.00 (reference)
CG	1.02 (0.74–1.41)	0.881	1.07 (0.77–1.49)	0.696
CC	0.66 (0.33–1.3)	0.226	0.95 (0.5–1.81)	0.879
C vs G	0.92 (0.72–1.18)	0.517	1.02 (0.79–1.3)	0.896
Dominant model	1.01 (0.92–1.11)	0.843	1.09 (0.63–1.89)	0.748
Recessive model	0.67 (0.38–1.2)	0.180	0.96 (0.7–1.31)	0.785
rs4586
CC	1.00 (reference)		1.00 (reference)
CT	1.54 (1.09–2.2)	0.016	1.43 (0.99–2.04)	0.054
TT	1.69 (1.07–2.67)	0.024	1.4 (0.87–2.27)	0.169
T vs C	1.33 (1.07–1.66)	0.011	1.22 (0.97–1.54)	0.089
Dominant model	1.64 (1.16–2.32)	0.005	1.48 (1.03–2.12)	0.035
Recessive model	1.36 (0.87–2.1)	0.176	1.18 (0.69–2.02)	0.537

Notes: *Adjusted for age, gender, BMI, and smoking status; bold values are statistically significant (P<0.05).

Figure 1 Associations between MCP-1 SNPs and circulating level of MCP1 in healthy controls.

Table 4 Distribution of Quartiles of MCP1 in COPD Cases and Controls

IFN-γ (pg/mL)	Cases	Controls	OR (95% CIs)*	P-value
Quartile 1 (≤126.8)	70	125	reference
Quartile 2 (126.8–157.1)	98	125	1.40 (0.94–2.08)	0.094
Quartile 3 (157.1–186.7)	98	125	1.40 (0.94–2.08)	0.094
Quartile 4 (>186.7)	184	125	2.63 (1.82–3.81)	<0.001
Per quartile			1.35 (1.21–1.52)	<0.001

Notes: *Adjusted for age, gender, BMI, and smoking status; bold values are statistically significant (P<0.05).