DNA Methylation Level of Transcription Factor Binding Site in the Promoter Region of Acyl-CoA Synthetase Family Member 3 (ACSF3) in Saudi Autistic Children

Figures & data

Table 1 Demographic Characteristics of ASD Children and Their Siblings

Case	Gender	Patient/Normal	Age (Years)	Birth	Severity of ASD	Onset of Symptoms	Family History
1	Male	P	12	Caesarean	Simple/level1	After 2 years	Yes
	Female	N	5
2	Male	P	9	Natural	Mild/level2	After 2 years	No
	Female	N	6
3	Male	P	10	Natural	Simple/level1	After 2 years	Not known
	Female	N	5
4	Male	P	8	Caesarean	Mild/level2	After 2 years	No
	Male	N	4
5	Male	P	8	Caesarean	Mild/level2	After 6 months	No
	Male	N	6
6	Male	P	6	Caesarean	Mild/level2	After 2 years	No
	Male	N	7
7	Male	P	7	Caesarean	Simple/level1	After 2 years	Yes
	Female	N	8
8	Male	P	8	Caesarean	Sever/level3	After 2 years	Yes
	Female	N	6
9	Male	P	7	Natural	Mild/level2	After first year	Yes
	Male	N	3
10	Male	P	12	Natural	Not specified	Not specified
	Male	N	10
11	Female	P	12	Caesarean	Mild/level2	After 2 years	No
	Male	N	10
12	Male	P	6	Natural	Simple/level1	After 2 years	Yes
	Female	N	11
13	Male	P	7	Natural	Simple/level1	After 2 years	No
	Female	N	5
14	Male	P	10	Caesarean	Mild/level2	After 2 years	No
	Male	N	6
15	Male	P	10	Natural	Simple/level1	After 2 years	No
	Female	N	7
16	Male	P	7	Caesarean	Simple/level1	After 2 years	No
	Male	N	5
17	Female	P	6	Caesarean	Simple/level1	After 2 years	No
	Female	N	4
18	Male	P	7	Natural	Simple/level1	After 2 years	No
	Female	P	8	Natural	simple/level1	After 2 years	No
	Male	N	7

Figure 1 The predicted CpG Island in the promoter of ACSF3. The island appears in the light blue region.

Table 2 ACSF3 and Sp1 Expressions in ASD Children

ACSF3 Expression in ASD Children				SP1 Expression in ASD Children
Upregulation		Downregulation		Upregulation		Downregulation
Sample	Fold Change	Sample	Fold Change	Sample	Fold Change	Sample	Fold Change
3	1.4	1	−2.1	3	1.5	1	−1.6
4	1.5	2	−2	4	1.8	2	−16.4
5	27.4	9	−2.2	5	14.7	7	−1.4
6	34.6	10	−1.7	6	46.6	8	−1.7
7	1.3	11	−1.4	15	1.5	9	−1.4
8	1.6	12	−1.2	16	176.8	10	−1.9
15	1.3	17	−1.6	17	3.5	11	−1.2
16	1.9	18	−1.7	18	245.1	12	−1.3
19	1.8		–			13	−1.1
						14	−13
						19	−1.2

Figure 2 The positive correlation between expression of ACSF3 and transcription factor (SP1) by SPSS software. (r = 0.454, P = 0.05).

Figure 3 Five dilutions of positive control (methylated human bisulfite-converted DNA).

Figure 4 Negative control (unmethylated human bisulfite-converted DNA).

Figure 5 Unconverted human genomic DNA.

Figure 6 Converted DNA samples. (A) Sample (1–40). (B) Samples (41–42).