Figures & data
Table 1. FC-CCD design with factors with corresponding levels and responses.
Figure 2. Cytotoxicity of DTX and CRM in MDA-MB-231 cells. Results are mean ± SEM (n = 3). p value significant at ***0.01.
![Figure 2. Cytotoxicity of DTX and CRM in MDA-MB-231 cells. Results are mean ± SEM (n = 3). p value significant at ***0.01.](/cms/asset/ab4955c1-b00a-4cf6-80ef-47a20a371407/idrd_a_1138339_f0002_c.jpg)
Figure 3. Solubility study of DTX (A), CRM (B) and 1:1 mixture of DTX and CRM (C) in solid lipids. Values are mean ± SD (n = 3).
![Figure 3. Solubility study of DTX (A), CRM (B) and 1:1 mixture of DTX and CRM (C) in solid lipids. Values are mean ± SD (n = 3).](/cms/asset/e7b9d851-0886-4a38-b365-5c7ab057d617/idrd_a_1138339_f0003_c.jpg)
Table 2. Results of FC-CCD experiment trials.
Table 3. Particle size, PDI and EE of SLN before and after freeze drying.
Figure 5. DSC characterization of SLN. (A) CRM, (B) DTX, (C) GMS, (D) Compritol, (E) blank SLN, (F) DC-SLN, (G) F-DC-SLN.
![Figure 5. DSC characterization of SLN. (A) CRM, (B) DTX, (C) GMS, (D) Compritol, (E) blank SLN, (F) DC-SLN, (G) F-DC-SLN.](/cms/asset/1d1068cc-a7d9-4216-ae69-f93fa82e37be/idrd_a_1138339_f0005_c.jpg)
Figure 8. FTIR characterization of folic acid on surface of SLN. (A) Folic acid, (B) blank SLN, (C) DC-SLN, (D) F-DC-SLN.
![Figure 8. FTIR characterization of folic acid on surface of SLN. (A) Folic acid, (B) blank SLN, (C) DC-SLN, (D) F-DC-SLN.](/cms/asset/10756a6e-2716-4226-b0a7-4bdb69d88e43/idrd_a_1138339_f0008_c.jpg)
Figure 9. In vitro release of F-DC-SLN in PBS (0.01 M, pH 7.4, 50% v/v ethanol). Values are mean ± SD (n = 3).
![Figure 9. In vitro release of F-DC-SLN in PBS (0.01 M, pH 7.4, 50% v/v ethanol). Values are mean ± SD (n = 3).](/cms/asset/0cc46629-3910-411d-8189-0049d1ce2433/idrd_a_1138339_f0009_c.jpg)
Figure 10. Cell uptake efficiency of SLN formulations. Values are mean ± SEM (n = 3). p value significant at *0.05.
![Figure 10. Cell uptake efficiency of SLN formulations. Values are mean ± SEM (n = 3). p value significant at *0.05.](/cms/asset/0e367241-6361-4515-b73e-1f8ad4f3e10c/idrd_a_1138339_f0010_c.jpg)
Figure 11. Fluoroscence microscope images shows internalization of free coumarin (a), DC-SLN (b) and F-DC-SLN (c) in MCF-7 cells.
![Figure 11. Fluoroscence microscope images shows internalization of free coumarin (a), DC-SLN (b) and F-DC-SLN (c) in MCF-7 cells.](/cms/asset/9ed41af7-8985-46ee-986d-a51e9ba6a725/idrd_a_1138339_f0011_c.jpg)
Figure 12. In vitro cytotoxicity of SLN in MCF-7 cells. Values are mean ± SEM (n = 3). p value significant at **0.01; ***0.001.
![Figure 12. In vitro cytotoxicity of SLN in MCF-7 cells. Values are mean ± SEM (n = 3). p value significant at **0.01; ***0.001.](/cms/asset/4d9f087d-a400-4934-b1f5-393be1dec270/idrd_a_1138339_f0012_c.jpg)
Figure 13. In vitro hemolysis test of SLN formulations. Values are mean ± SD (n = 3). p value significant at ***0.001.
![Figure 13. In vitro hemolysis test of SLN formulations. Values are mean ± SD (n = 3). p value significant at ***0.001.](/cms/asset/adb63b62-90a1-4283-9b55-be22be1526fa/idrd_a_1138339_f0013_c.jpg)
Figure 14. Plasma concentrations versus time profile of DTX after i.v. administration of DTX formulations. Values are mean ± SD (n = 5).
![Figure 14. Plasma concentrations versus time profile of DTX after i.v. administration of DTX formulations. Values are mean ± SD (n = 5).](/cms/asset/a9195499-1f2d-415a-a899-05e92871e476/idrd_a_1138339_f0014_c.jpg)