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Artificial Cells, Blood Substitutes, and Biotechnology Volume 40, 2012 - Issue 5
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Research Article

Glucose biosensor based on the immobilization of glucose oxidase on electrochemically synthesized polypyrrole-poly(vinyl sulphonate) composite film by cross-linking with glutaraldehyde

Özlem ÇolakDepartment of Chemistry, Faculty of Science, Gazi University, Ankara, TurkeyCorrespondence[email protected]
,
Ahmet YaşarDepartment of Chemistry, Faculty of Science, Gazi University, Ankara, Turkey
,
Servet ÇeteDepartment of Chemistry, Faculty of Science, Gazi University, Ankara, Turkey
&
Fatma ArslanDepartment of Chemistry, Faculty of Science, Gazi University, Ankara, Turkey
Pages 354-361 | Received 23 Jan 2012, Accepted 19 Mar 2012, Published online: 30 Apr 2012

Figures & data

Figure 1. Schematic diagram of GOD reaction; glucose oxidase catalyzes the oxidation of β-D-glucose by molecular oxygen to δ-gluconolactone.

Figure 1. Schematic diagram of GOD reaction; glucose oxidase catalyzes the oxidation of β-D-glucose by molecular oxygen to δ-gluconolactone.

Figure 2. Scanning electron micrographs of PPy/PVS electrode surface: (a) without enzyme; (b) with enzyme.

Figure 2. Scanning electron micrographs of PPy/PVS electrode surface: (a) without enzyme; (b) with enzyme.

Figure 3. Effect of the amount of glutaraldehyde on the amperometric response of glucose biosensor (0.1 M, pH 7.5 phosphate buffer, 25°C).

Figure 3. Effect of the amount of glutaraldehyde on the amperometric response of glucose biosensor (0.1 M, pH 7.5 phosphate buffer, 25°C).

Table I. Effect of the amount of glutaraldehyde on the reproducibility of glucose biosensor.

Figure 4. Effect of the working potential on the biosensor response (0.1 M, pH 7.5 phosphate buffer, 25°C).

Figure 4. Effect of the working potential on the biosensor response (0.1 M, pH 7.5 phosphate buffer, 25°C).

Figure 5. Effect of the pH on the biosensor response (phosphate buffer, 25°C).

Figure 5. Effect of the pH on the biosensor response (phosphate buffer, 25°C).

Figure 6. Effect of the temperature on the biosensor response (0.1 M, pH 7.5 phosphate buffer).

Figure 6. Effect of the temperature on the biosensor response (0.1 M, pH 7.5 phosphate buffer).

Figure 7. The effect of glucose concentration upon the amperometric response of the biosensor (Michealis-Menten plot, in pH 7.5 phosphate buffer; operating potential is +0.4 V, 25°C).

Figure 7. The effect of glucose concentration upon the amperometric response of the biosensor (Michealis-Menten plot, in pH 7.5 phosphate buffer; operating potential is +0.4 V, 25°C).

Figure 8. The calibration curves of the glucose biosensor ranging between (a) 1.0× 10-6-5×10-5M; (b) 5×10-5M-1mM (0.1 M, pH 7.5 phosphate buffer, 25°C).

Figure 8. The calibration curves of the glucose biosensor ranging between (a) 1.0× 10-6-5×10-5M; (b) 5×10-5M-1mM (0.1 M, pH 7.5 phosphate buffer, 25°C).

Figure 9. The effect of glucose concentration upon the amperometric response of the biosensor (Lineweaver-Burk plot, in pH 7.5 phosphate buffer and at a 0.4 V operating potential, 25°C).

Figure 9. The effect of glucose concentration upon the amperometric response of the biosensor (Lineweaver-Burk plot, in pH 7.5 phosphate buffer and at a 0.4 V operating potential, 25°C).

Figure 10. Reuse number of the enzyme electrode (5.0 10-5M glucose concentration, 0.1 M pH 7.5 phosphate buffer, 25°C).

Figure 10. Reuse number of the enzyme electrode (5.0 10-5M glucose concentration, 0.1 M pH 7.5 phosphate buffer, 25°C).

Figure 11. Storage stabilization of the enzyme electrode (0.1 M, pH 7.5 phosphate buffer, 25°C).

Figure 11. Storage stabilization of the enzyme electrode (0.1 M, pH 7.5 phosphate buffer, 25°C).

Table II. Effect of potential interferants on the glucose biosensor.

Table III. Determination of glucose in blood serum samples.

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