Isolation and biological activity of compounds from Garcinia preussii

Figures & data

Figure 1. Structures of compounds 1–8.

Table 1. Activities of compounds 1–8 in DPPH^•, ABTS^•−, ALP, and ORAC assays.

Compounds	DPPH^• (ER50)	ABTS^•− (ER50)	ALP (EC50 µM)	ORAC (EC50 µM)
1	0.20 ± 0.03	0.19 ± 0.02	9.72 ± 2.40	6.04 ± 0.36
2	0.21 ± 0.02	0.20 ± 0.01	8.23 ± 1.23	5.72 ± 0.40
3	0.45 ± 0.11	0.29 ± 0.02	5.22 ± 1.72	7.57 ± 0.17
4	0.11 ± 0.01	0.09 ± 0.01	nd	3.24 ± 0.28
6	>1	>1	9.31 ± 2.70	23.1 ± 2.10
7	>1	0.70 ± 0.03	2.30 ± 0.25	2.96 ± 0.27
8	0.62 ± 0.03	0.65 ± 0.03	4.53 ± 0.71	4.75 ± 0.46
Quercetin	0.09 ± 0.01	0.07 ± 0.01	1.00 ± 0.07	1.66 ± 0.07

Results are the means of at least three independent determinations ± SD. ER₅₀: concentration inducing a 50% decrease in absorbance after 90 min. nd, not determined.

Table 2. Minimum inhibitory concentration (MIC) of compounds 1–8.

	MIC (µg/mL)
Compounds	S. aureus	E. faecalis
1	64	128
2	>128	128
3	>128	128
4	16	16
5	2	2
6	>128	>128
7	>128	>128
8	>128	>128
Gentamicin	1	16

Results are based on at least three independent determinations.

Table 3. Cytotoxicity (IC₅₀ in µM) of isolated benzophenones (1–6) in human cancer cell lines.

Compounds	DU145	HeLa	HT-29	A431
1	7.7 ± 1.4	14.6 ± 7.2	7.4 ± 0.2	9.9 ± 2.0
2	14.5 ± 3.2	21.0 ± 5.9	15.9 ± 4.1	17.1 ± 3.5
3	11.5 ± 2.0	13.0 ± 3.7	14.0 ± 3.0	11.0 ± 3.5
4	14.9 ± 0.8	16.4 ± 4.0	16.1 ± 3.7	9.9 ± 1.0
5	33.4 ± 5.2	49.5 ± 15.1	32.6 ± 1.7	38.2 ± 1.1
6	>150	>150	>150	>150
Colchicine	0.0230 ± 0.0008	0.0060 ± 0.0014	0.0140 ± 0.0021	0.0060 ± 0.0003

Compounds were evaluated for cytotoxicity by standard procedures as described in the Experimental section. The following cultured human cell lines were employed: DU145 (prostate carcinoma), HeLa (cervical cancer), HT-29 (colorectal cancer), and A431 (epidermoid carcinoma). Results are shown as IC₅₀ values (µM) and are the means of at least three independent determinations ± SD.