Figures & data
Table 1. Purification scheme of GST from rainbow trout erythrocytes.
Table 2. Ki and IC50 values obtained from regression analysis graphs for rainbow trout erythrocytes GST in the presence of different metal ion concentrations.
Figure 1. SDS-PAGE photograph. Lane 1: Rainbow trout erythrocytes GST. Lane 2: Standard proteins: rabbit phosphorylase B (97 kDa), bovine albumin (66 kDa), chicken ovalbumin (45 kDa), bovine erythrocyte carbonic anhydrase (29 kDa).
![Figure 1. SDS-PAGE photograph. Lane 1: Rainbow trout erythrocytes GST. Lane 2: Standard proteins: rabbit phosphorylase B (97 kDa), bovine albumin (66 kDa), chicken ovalbumin (45 kDa), bovine erythrocyte carbonic anhydrase (29 kDa).](/cms/asset/a2221947-7ba5-4fe7-a078-25b3f04c1e75/ienz_a_729829_f0001_b.gif)
Figure 2. Standard Rf-Log MW graph of GST using SDS-PAGE. (Standards: Rabbit phosphorylase B (97.4 kDa), bovine albumin (66 kDa), chicken ovalbumin (45 kDa), and bovine carbonic anhydrase (29 kDa).
![Figure 2. Standard Rf-Log MW graph of GST using SDS-PAGE. (Standards: Rabbit phosphorylase B (97.4 kDa), bovine albumin (66 kDa), chicken ovalbumin (45 kDa), and bovine carbonic anhydrase (29 kDa).](/cms/asset/324c2cca-6371-456a-8248-bd1cc0ead46d/ienz_a_729829_f0002_b.gif)
Figure 3. Standard Kav-Log MW graph of GST using gel filtration. (Standards: Cytochrome c (12 kDa), bovine erythrocyte carbonic anhydrase (29 kDa), yeast alcohol dehydrogenase (150 kDa) and β-amylase (200 kDa).
![Figure 3. Standard Kav-Log MW graph of GST using gel filtration. (Standards: Cytochrome c (12 kDa), bovine erythrocyte carbonic anhydrase (29 kDa), yeast alcohol dehydrogenase (150 kDa) and β-amylase (200 kDa).](/cms/asset/2a7abf6a-2934-4216-b1e2-e46a92d479fa/ienz_a_729829_f0003_b.gif)
Figure 8. Lineweaver-Burk graph in 5 different GSH concentrations and in constant CDNB concentration.
![Figure 8. Lineweaver-Burk graph in 5 different GSH concentrations and in constant CDNB concentration.](/cms/asset/82af5082-4f63-432e-a424-edbe55572625/ienz_a_729829_f0008_b.gif)
Figure 9. Lineweaver-Burk graph in 5 different CDNB concentrations and in constant GSH concentration.
![Figure 9. Lineweaver-Burk graph in 5 different CDNB concentrations and in constant GSH concentration.](/cms/asset/141b4495-89a5-41d5-a64a-ccc4d1989c37/ienz_a_729829_f0009_b.gif)